Virtual reality assisted microscopy data visualization and colocalization analysis

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dc.contributor.authorTheart, Rensu P.en_ZA
dc.contributor.authorLoos, Benen_ZA
dc.contributor.authorNiesler, Thomas R.en_ZA
dc.date.accessioned2017-02-24T07:36:49Z
dc.date.available2017-02-24T07:36:49Z
dc.date.issued2017-02-15
dc.date.updated2017-02-24T07:02:35Z
dc.descriptionCITATION: Theart, R. P., Loos, B. & Niesler, T. R. 2016. Virtual reality assisted microscopy data visualization and colocalization analysis. BMC Bioinformatics, 18(2):64, doi:10.1186/s12859-016-1446-2.
dc.descriptionThe original publication is available at https://bmcbioinformatics.biomedcentral.com
dc.description.abstractBackground: Confocal microscopes deliver detailed three-dimensional data and are instrumental in biological analysis and research. Usually, this three-dimensional data is rendered as a projection onto a two-dimensional display. We describe a system for rendering such data using a modern virtual reality (VR) headset. Sample manipulation is possible by fully-immersive hand-tracking and also by means of a conventional gamepad. We apply this system to the specific task of colocalization analysis, an important analysis tool in biological microscopy. We evaluate our system by means of a set of user trials. Results: The user trials show that, despite inaccuracies which still plague the hand tracking, this is the most productive and intuitive interface. The inaccuracies nevertheless lead to a perception among users that productivity is low, resulting in a subjective preference for the gamepad. Fully-immersive manipulation was shown to be particularly effective when defining a region of interest (ROI) for colocalization analysis. Conclusions: Virtual reality offers an attractive and powerful means of visualization for microscopy data. Fully immersive interfaces using hand tracking show the highest levels of intuitiveness and consequent productivity. However, current inaccuracies in hand tracking performance still lead to a disproportionately critical user perception.en_ZA
dc.description.urihttps://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-016-1446-2
dc.description.versionPublisher's version
dc.format.extent16 pagesen_ZA
dc.identifier.citationTheart, R. P., Loos, B. & Niesler, T. R. 2016. Virtual reality assisted microscopy data visualization and colocalization analysis. BMC Bioinformatics, 18(2):64, doi:10.1186/s12859-016-1446-2
dc.identifier.issn1471-2105 (online)
dc.identifier.otherdoi:10.1186/s12859-016-1446-2
dc.identifier.urihttp://hdl.handle.net/10019.1/100640
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Central
dc.rights.holderAuthors retain copyright
dc.subjectVirtual reality in engineeringen_ZA
dc.subjectConfocal microscopyen_ZA
dc.subjectBiology -- Microscopyen_ZA
dc.subjectThree-dimensional imagingen_ZA
dc.titleVirtual reality assisted microscopy data visualization and colocalization analysisen_ZA
dc.typeArticle
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