SDS interferes with SaeS signalling of Staphylococcus aureus independently of SaePQ

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dc.contributor.authorMakgotlho, Phuti E.en_ZA
dc.contributor.authorMarincola, Gabriellaen_ZA
dc.contributor.authorSchafer, Danielen_ZA
dc.contributor.authorLiu, Qianen_ZA
dc.contributor.authorBae, Taeoken_ZA
dc.contributor.authorGeiger, Tobiasen_ZA
dc.contributor.authorWasserman, Elizabethen_ZA
dc.contributor.authorWolz, Christianeen_ZA
dc.contributor.authorZiebuhr, Wilmaen_ZA
dc.contributor.authorSinha, Bhanuen_ZA
dc.date.accessioned2014-07-07T12:26:20Z
dc.date.available2014-07-07T12:26:20Z
dc.date.issued2013-08-20
dc.descriptionCITATION: Makgotlho, P. E. et al. 2013. SDS interferes with SaeS signalling of Staphylococcus aureus independently of SaePQ. PLoS ONE, 8(8): e71644, doi:10.1371/journal.pone.0071644.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractThe Staphylococcus aureus regulatory saePQRS system controls the expression of numerous virulence factors, including extracellular adherence protein (Eap), which amongst others facilitates invasion of host cells. The saePQRS operon codes for 4 proteins: the histidine kinase SaeS, the response regulator SaeR, the lipoprotein SaeP and the transmembrane protein SaeQ. S. aureus strain Newman has a single amino acid substitution in the transmembrane domain of SaeS (L18P) which results in constitutive kinase activity. SDS was shown to be one of the signals interfering with SaeS activity leading to inhibition of the sae target gene eap in strains with SaeSL but causing activation in strains containing SaeSP. Here, we analyzed the possible involvement of the SaeP protein and saePQ region in SDS-mediated sae/eap expression. We found that SaePQ is not needed for SDS-mediated SaeS signaling. Furthermore, we could show that SaeS activity is closely linked to the expression of Eap and the capacity to invade host cells in a number of clinical isolates. This suggests that SaeS activity might be directly modulated by structurally non-complex environmental signals, as SDS, which possibly altering its kinase/phosphatase activity.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071644
dc.description.versionPublisher's version
dc.format.extent9 pages
dc.identifier.citationMakgotlho, P. E. et al. 2013. SDS interferes with SaeS signalling of Staphylococcus aureus independently of SaePQ. PLoS ONE, 8(8): e71644, doi:10.1371/journal.pone.0071644.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0071644
dc.identifier.urihttp://hdl.handle.net/10019.1/93511
dc.language.isoen_ZA
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectStaphylococcus aureusen_ZA
dc.subjectSDSen_ZA
dc.subjectProteinsen_ZA
dc.subjectVirulence (Microbiology)en_ZA
dc.subjectSaePQRS operonen_ZA
dc.subjectMicrobial invasivenessen_ZA
dc.titleSDS interferes with SaeS signalling of Staphylococcus aureus independently of SaePQen_ZA
dc.typeArticle
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