Interferon-gamma release assays for the detection of Mycobacterium tuberculosis infection in children with cancer

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dc.contributor.authorStefan D.C.
dc.contributor.authorDippenaar A.
dc.contributor.authorDetjen A.K.
dc.contributor.authorSchaaf H.S.
dc.contributor.authorMarais B.J.
dc.contributor.authorKriel B.
dc.contributor.authorLoebenberg L.
dc.contributor.authorWalzl G.
dc.contributor.authorHesseling A.C.
dc.date.accessioned2011-05-15T16:16:45Z
dc.date.available2011-05-15T16:16:45Z
dc.date.issued2010
dc.description.abstractSETTING: The paediatric oncology unit at Tygerberg Children's Hospital, South Africa. OBJECTIVES: To assess the use of the tuberculin skin test (TST) and two commercial interferon-gamma release assays (IGRAs) for the detection of Mycobacterium tuberculosis infection in children with cancer before initiating chemotherapy treatment. DESIGN: Prospective hospital-based study, including children newly diagnosed with cancer; all underwent TST and IGRA testing. RESULTS: Of the 34 children enrolled, seven (17.6%) tested positive with either test: TST (3/7, 8.8%), TSPOT®.TB (n = 6, 17.6%) and QuantiFERON®-TB Gold In-Tube (QFT-G; n = 3, 8.8%). T-SPOT.TB assay results were negative in 17 (50.0%) and indeterminate in four (11.8%) children. Six T-SPOT.TB tests could not be completed due to low cell counts (<100 000 per well), and one clotted. QFT-G results were negative in 26 (76.5%) and indeterminate in five (14.7%). CONCLUSIONS: TST and IGRAs were frequently discordant, with fewer positive results than expected. T-SPOT. TB produced more positive results, but inadequate cell counts were a particular problem. The sample size was too small to comment with confidence on test accuracy. All latent TB infection tests appear to perform subo ptimally in this group of children, and therefore none of them can be used in isolation to confirm or disprove TB infection. © 2010 The Union.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Tuberculosis and Lung Disease
dc.identifier.citation14
dc.identifier.citation6
dc.identifier.issn10273719
dc.identifier.urihttp://hdl.handle.net/10019.1/13920
dc.subjectgamma interferon
dc.subjectisoniazid
dc.subjectrifampicin
dc.subjectacute lymphoblastic leukemia
dc.subjectacute myeloblastic leukemia
dc.subjectadolescent
dc.subjectantibiotic resistance
dc.subjectarticle
dc.subjectblood cell count
dc.subjectbrain tumor
dc.subjectBurkitt lymphoma
dc.subjectcancer chemotherapy
dc.subjectchild
dc.subjectchildhood cancer
dc.subjectclear cell sarcoma
dc.subjectclinical article
dc.subjectclinical trial
dc.subjectcontrolled clinical trial
dc.subjectcontrolled study
dc.subjectcytokine release
dc.subjectenzyme linked immunosorbent assay
dc.subjectenzyme linked immunospot assay
dc.subjectfemale
dc.subjectgastric suction
dc.subjecthistiocytosis
dc.subjectHodgkin disease
dc.subjecthospital based case control study
dc.subjecthuman
dc.subjectimmunoassay
dc.subjectinfant
dc.subjectinterferon gamma release assay
dc.subjectleukocyte count
dc.subjectleukocyte differential count
dc.subjectliver function test
dc.subjectlymphocyte count
dc.subjectlymphocytopenia
dc.subjectmale
dc.subjectMycobacterium tuberculosis
dc.subjectneuroblastoma
dc.subjectosteosarcoma
dc.subjectpatient identification
dc.subjectphlebotomy
dc.subjectpreschool child
dc.subjectpriority journal
dc.subjectprospective study
dc.subjectprotein determination
dc.subjectrhabdomyosarcoma
dc.subjectschool child
dc.subjectsingle blind procedure
dc.subjectsputum culture
dc.subjectT cell lymphoma
dc.subjectthorax radiography
dc.subjecttuberculin test
dc.subjecttuberculosis
dc.subjectAdolescent
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectDiagnosis, Differential
dc.subjectFemale
dc.subjectFollow-Up Studies
dc.subjectHumans
dc.subjectIncidence
dc.subjectInfant
dc.subjectInterferon-gamma
dc.subjectLatent Tuberculosis
dc.subjectMale
dc.subjectNeoplasms
dc.subjectProspective Studies
dc.subjectReproducibility of Results
dc.subjectSouth Africa
dc.subjectTime Factors
dc.titleInterferon-gamma release assays for the detection of Mycobacterium tuberculosis infection in children with cancer
dc.typeArticle
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