Application of Caco-2 cell line in herb-drug interaction studies : current approaches and challenges
| dc.contributor.author | Awortwe, C. | en_ZA |
| dc.contributor.author | Fasinu, P. S. | en_ZA |
| dc.contributor.author | Rosenkranz, B. | en_ZA |
| dc.date.accessioned | 2016-07-06T12:13:53Z | |
| dc.date.available | 2016-07-06T12:13:53Z | |
| dc.date.issued | 2014-01 | |
| dc.description | CITATION: Awortwe, C., Fasinu, P. S. & Rozenkranz, B. 2014. Application of Caco-2 cell line in herb-drug interaction studies: current approaches and challenges. Journal of Pharmacy and Pharmaceutical Sciences, 17(1):1-19, doi: 10.18433/J30K63. | |
| dc.description | The original publication is available at https://ejournals.library.ualberta.ca/index.php/JPPS/index | |
| dc.description.abstract | The Caco-2 model is employed in pre-clinical investigations to predict the likely gastrointestinal permeability of drugs because it expresses cytochrome P450 enzymes, transporters, microvilli and enterocytes of identical characteristics to the human small intestine. The FDA recommends this model as integral component of the Biopharmaceutics Classification System (BCS). Most dedicated laboratories use the Caco-2 cell line to screen new chemical entities through prediction of its solubility, bioavailability and the possibility of drug-drug or herb-drug interactions in the gut lumen. However, challenges in the inherent characteristics of Caco-2 cell and inter-laboratory protocol variations have resulted to generation of irreproducible data. These limitations affect the extrapolation of data from pre-clinical research to clinical studies involving drug-drug and herb-drug interactions. This review addresses some of these caveats and enumerates the plausible current and future approaches to reduce the anomalies associated with Caco-2 cell line investigations focusing on its application in herb-drug interactions. | en_ZA |
| dc.description.version | Publisher's version | |
| dc.format.extent | 19 pages | |
| dc.identifier.citation | Awortwe, C., Fasinu, P. S. & Rozenkranz, B. 2014. Application of Caco-2 cell line in herb-drug interaction studies: current approaches and challenges. Journal of Pharmacy and Pharmaceutical Sciences, 17(1):1-19, doi: 10.18433/J30K63. | |
| dc.identifier.issn | 1482-1826 (online) | |
| dc.identifier.other | doi: 10.18433/J30K63 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/99096 | |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | Canadian Society for Pharmaceutical Sciences | |
| dc.rights.holder | Journal of Pharmacy and Pharmaceutical Sciences | |
| dc.subject | Caco-2 model | en_ZA |
| dc.subject | Biopharmaceutics Classification System | en_ZA |
| dc.subject | Herb-drug interactions | en_ZA |
| dc.title | Application of Caco-2 cell line in herb-drug interaction studies : current approaches and challenges | en_ZA |
| dc.type | Article | en_ZA |