Characterisation of different GLRaV-3 variant infections by determining virus concentration ratios and miRNA expression profiles
| dc.contributor.author | Aldrich, D. J. | en_ZA |
| dc.contributor.author | Bester, R. | en_ZA |
| dc.contributor.author | Burger, J. | en_ZA |
| dc.contributor.author | Maree, H. J. | en_ZA |
| dc.date.accessioned | 2021-01-21T06:57:55Z | |
| dc.date.available | 2021-01-21T06:57:55Z | |
| dc.date.issued | 2019 | |
| dc.description | CITATION: Aldrich, D. J., et. al. 2019. Characterisation of different GLRaV-3 variant infections by determining virus concentration ratios and miRNA expression profiles. Vitis: Journal of Grapevine Research, 58:79–86, doi:10.5073/vitis.2019.58.79-86. | |
| dc.description | The original publication is available at https://ojs.openagrar.de/index.php/VITIS | |
| dc.description.abstract | Grapevine leafroll disease (GLD) is present in all grape-growing regions of the world and is considered the most significant grapevine viral disease. Grapevine leafroll-associated virus 3 (GLRaV-3) is considered the primary cause of GLD and in South African vineyards five genetic variant groups (I, II, III, VI and VII) have been confirmed. Biological distinctions between GLRaV-3 variants have not been fully validated. By characterising virus concentration and stress-responsive microRNA expression in GLRaV-3 infected plants, this study aimed to glean a better understanding of the possible biological distinctions between GLRaV-3 variants. Quantitative reverse transcription PCR was utilised for virus concentration ratio (VCR) determination and miRNA quantitation in GLRaV-3 positive and negative grapevines grown under greenhouse and field conditions. This study found statistically significant differences in VCRs in plants singly infected with different GLRaV-3 variants. Interestingly, no difference in mean VCRs were observed between data sets, despite notable differences in plant age, duration of GLRaV-3 infection, scion/rootstock combination and growing conditions. Several miRNAs showed statistically significant expression modulation between infected and healthy samples. miRNA expression between data sets varied substantially and a greater overall miRNA response was observed in plants with more established GLRaV-3 infections. The lack of significant differences in mean VCRs between data sets, coupled with the consistent modulation of certain miRNAs in plants that have likely been infected for longer is a promising result. This finding could indicate that successful inhibition of further virus replication by plant defence mechanisms occurred, and that these miRNAs are implicated in this response. | en_ZA |
| dc.description.uri | https://ojs.openagrar.de/index.php/VITIS/article/view/10703 | |
| dc.description.version | Publisher's version | |
| dc.format.extent | 8 pages | |
| dc.identifier.citation | Aldrich, D. J., et. al. 2019. Characterisation of different GLRaV-3 variant infections by determining virus concentration ratios and miRNA expression profiles. Vitis: Journal of Grapevine Research, 58:79–86, doi:10.5073/vitis.2019.58.79-86 | |
| dc.identifier.issn | 2367-4156 (online) | |
| dc.identifier.issn | 0042-7500 (print) | |
| dc.identifier.other | doi:10.5073/vitis.2019.58.79-86 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/109006 | |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | Bundesanstalt fur Zuchtungsforschung an Kulturpflanzen | |
| dc.rights.holder | Authors retain copyright | |
| dc.subject | Grapevine leafroll disease | en_ZA |
| dc.subject | Grapevine leafroll virus -- South Africa | en_ZA |
| dc.subject | Grapes -- Disease and pest resistance -- Genetic aspects | en_ZA |
| dc.subject | Cabernet Sauvignon | en_ZA |
| dc.subject | Cabernet (Wine) | en_ZA |
| dc.subject | Wine and wine making | en_ZA |
| dc.subject | MicroRNA -- Analysis | en_ZA |
| dc.title | Characterisation of different GLRaV-3 variant infections by determining virus concentration ratios and miRNA expression profiles | en_ZA |
| dc.type | Article | en_ZA |