Immunohistomorphology of pancreatic islet microvasculature and the immunophenotypic analysis of CEPC in adult diabetic rats

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dc.contributor.authorTchokonte-Nana, Venanten_ZA
dc.contributor.authorLe Roux, Danie Jacobusen_ZA
dc.contributor.authorKotze, Patricia Claraen_ZA
dc.contributor.authorNgounou, Eleonoreen_ZA
dc.date.accessioned2019-01-28T07:11:24Z
dc.date.available2019-01-28T07:11:24Z
dc.date.issued2017
dc.descriptionCITATION: Tchokonte-Nana, V. 2017. Immunohistomorphology of pancreatic islet microvasculature and the immunophenotypic analysis of CEPC in adult diabetic rats. International journal of morphology, 35(4):1560-1567.
dc.descriptionThe original publication is available at http://www.intjmorphol.com
dc.description.abstractENGLISH ABSTRACT: Hyperglycaemia is one of the main causes for the endothelial cell (EC) damage in diabetic patients. Even though circulating endothelial progenitor cells (CEPC) could be used as a prognosis for microvascular complications, there is very little information on the islet microvasculature. We analysed by immunohistochemistry and by flow cytometric immunophenotyping, the expression of CD34 on EC and the expressions of CD31, CD34, CD45 and CD133 on CEPC in Streptozotocin (STZ)-induced diabetic rats. Peripheral blood and tissue specimens were obtained from rats of different treatment regimens: STZ treatment, control saline (NS) and sodium citrate (CB) treatments. Blood cells were exposed to flow cytometric immunophenotyping for CD133, CD31, CD34, CD45 and CD133. While tissues from the pancreas, liver and kidney were routinely processed and stained immunohistochemically for CD34. There was a tendency of an increased in CD45-/CD133+/CD31+/CD34+ cells (0.04 ± 0.11 %) in diabetic rats compared to the controls (CB: 0.03 ± 0.04 %; Saline: 0.01 ± 0.03 %). But there was no significant statistical difference between them. The expression pattern of CD34 on the EC in the organs’ vascular beds including arterioles, venules, capillaries and sinusoids was extremely heterogeneous across and within treatment regimens. The ECs in the sinusoids of the liver presented similar CD34 expression patterns across different treatment regimens, while the expression of CD34 on the ECs of sinusoidal capillaries in the pancreas vary with the treatment regimen. We conclude that the degree of endothelial cell damage is not uniform across organs’ vascular beds in the rat, contrary to mice and humans. Furthermore, the sinusoids in the pancreas and the kidney may have the same degree of endothelial damage when exposed to the same deleterious causes.en_ZA
dc.description.urihttp://www.intjmorphol.com/abstract/?art_id=4601
dc.description.versionPublisher's version
dc.format.extent8 pagesen_ZA
dc.identifier.citationTchokonte-Nana, V. 2017. Immunohistomorphology of pancreatic islet microvasculature and the immunophenotypic analysis of CEPC in adult diabetic rats. International journal of morphology, 35(4):1560-1567
dc.identifier.issn0717-9502 (online)
dc.identifier.issn0717-9367 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/105364
dc.language.isoen_ZAen_ZA
dc.publisherSociedad Chilena de Anatomiaen_ZA
dc.rights.holderSociedad Chilena de Anatomíaen_ZA
dc.subjectPancreasen_ZA
dc.subjectPancreatic isletsen_ZA
dc.subjectVasculatureen_ZA
dc.subjectImmunophenotypingen_ZA
dc.titleImmunohistomorphology of pancreatic islet microvasculature and the immunophenotypic analysis of CEPC in adult diabetic ratsen_ZA
dc.typeArticleen_ZA
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