Evidence for novel hepaciviruses in rodents

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dc.contributor.authorDrexler, Jan Felixen_ZA
dc.contributor.authorCorman, Victor Maxen_ZA
dc.contributor.authorMuller, Marcel Alexanderen_ZA
dc.contributor.authorLukashev, Alexander N.en_ZA
dc.contributor.authorGmyl, Anatolyen_ZA
dc.contributor.authorCoutard, Brunoen_ZA
dc.contributor.authorAdam, Alexanderen_ZA
dc.contributor.authorRitz, Danielen_ZA
dc.contributor.authorLeijten, Lonneke M.en_ZA
dc.contributor.authorMatthee, Sonjaen_ZA
dc.contributor.authorVan Riel, Debbyen_ZA
dc.contributor.authorKallies, Reneen_ZA
dc.contributor.authorKlose, Stefan M.en_ZA
dc.contributor.authorGloza-Rausch, Florianen_ZA
dc.contributor.authorBinger, Tabeaen_ZA
dc.contributor.authorAnnan, Augustinaen_ZA
dc.contributor.authorAdu-Sarkodie, Yawen_ZA
dc.contributor.authorOppong, Samuelen_ZA
dc.contributor.authorBourgarel, Mathieuen_ZA
dc.contributor.authorRupp, Danielen_ZA
dc.contributor.authorHoffmann, Bernden_ZA
dc.contributor.authorSchlegel, Mathiasen_ZA
dc.contributor.authorKummerer, Beate M.en_ZA
dc.contributor.authorKruger, Detlev H.en_ZA
dc.contributor.authorSchmidt-Chanasit, Jonasen_ZA
dc.contributor.authorSetien, Alvaro Aguilaren_ZA
dc.contributor.authorCottontail, Veronika M.en_ZA
dc.contributor.authorHemachudha, Thiravaten_ZA
dc.contributor.authorWacharapluesadee, Supapornen_ZA
dc.contributor.authorOsterrieder, Klausen_ZA
dc.contributor.authorBartenschlager, Ralfen_ZA
dc.contributor.authorBeer, Martinen_ZA
dc.contributor.authorKuiken, Thijsen_ZA
dc.contributor.authorReusken, Chantalen_ZA
dc.contributor.authorLeroy, Eric M.en_ZA
dc.contributor.authorUlrich, Rainer G.en_ZA
dc.contributor.authorDrosten, Christianen_ZA
dc.date.accessioned2014-07-07T12:33:47Z
dc.date.available2014-07-07T12:33:47Z
dc.date.issued2013-06-20
dc.descriptionCITATION: Drexler, J. F. et al. 2013. Evidence for novel hepaciviruses in rodents. PLoS Pathogens, 9(6): e1003438, doi:10.1371/journal.ppat.1003438.
dc.descriptionThe original publication is available at http://journals.plos.org/plospathogens
dc.description.abstractHepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research is complicated by a lack of accessible small animal models. The systematic investigation of viruses of small mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled the so-far largest collection of small-mammal samples from around the world, qualified to be screened for bloodborne viruses, including sera and organs from 4,770 rodents (41 species); and sera from 2,939 bats (51 species). Three highly divergent rodent hepacivirus clades were detected in 27 (1.8%) of 1,465 European bank voles (Myodes glareolus) and 10 (1.9%) of 518 South African four-striped mice (Rhabdomys pumilio). Bats showed anti-HCV immunoblot reactivities but no virus detection, although the genetic relatedness suggested by the serologic results should have enabled RNA detection using the broadly reactive PCR assays developed for this study. 210 horses and 858 cats and dogs were tested, yielding further horse-associated hepaciviruses but none in dogs or cats. The rodent viruses were equidistant to HCV, exceeding by far the diversity of HCV and the canine/equine hepaciviruses taken together. Five full genomes were sequenced, representing all viral lineages. Salient genome features and distance criteria supported classification of all viruses as hepaciviruses. Quantitative RT-PCR, RNA in-situ hybridisation, and histopathology suggested hepatic tropism with liver inflammation resembling hepatitis C. Recombinant serology for two distinct hepacivirus lineages in 97 bank voles identified seroprevalence rates of 8.3 and 12.4%, respectively. Antibodies in bank vole sera neither cross-reacted with HCV, nor the heterologous bank vole hepacivirus. Co-occurrence of RNA and antibodies was found in 3 of 57 PCR-positive bank vole sera (5.3%). Our data enable new hypotheses regarding HCV evolution and encourage efforts to develop rodent surrogate models for HCV.en_ZA
dc.description.urihttp://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003438
dc.description.versionPublisher's version
dc.format.extent17 pages
dc.identifier.citationDrexler, J. F. et al. 2013. Evidence for novel hepaciviruses in rodents. PLoS Pathogens, 9(6): e1003438, doi:10.1371/journal.ppat.1003438.
dc.identifier.issn1553-7374 (online)
dc.identifier.otherdoi:10.1371/journal.ppat.1003438
dc.identifier.urihttp://hdl.handle.net/10019.1/94901
dc.language.isoen_ZA
dc.publisherPLoS
dc.rights.holderAuthors retain copyright
dc.subjectHepatitis C virusen_ZA
dc.subjectHepacivirusesen_ZA
dc.subjectViruses -- Evolutionen_ZA
dc.subjectRodents as laboratory animalsen_ZA
dc.titleEvidence for novel hepaciviruses in rodentsen_ZA
dc.typeArticle
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