The effect of ultraviolet-C treatment on the biochemical composition of beer
dc.contributor.advisor | Swart, Pieter | en_ZA |
dc.contributor.advisor | Rautenbach, Marina | en_ZA |
dc.contributor.author | Mfa-Mezui, Antoine Aime | en_ZA |
dc.contributor.other | Stellenbosch University. Faculty of Science. Dept. of Biochemistry. | en_ZA |
dc.date.accessioned | 2012-02-10T13:20:32Z | en_ZA |
dc.date.accessioned | 2012-03-30T11:07:45Z | |
dc.date.available | 2012-11-30T22:10:03Z | |
dc.date.issued | 2012-03 | en_ZA |
dc.description | Thesis (PhD)--Stellenbosch University, 2012. | en_ZA |
dc.description.abstract | ENGLISH ABSTRACT: This study describes: · Development of analytical tools to investigate the light struck flavour (LSF) in beer by Gas chromatography mass spectrometry (GCMS) and by liquid chromatography mass spectrometry/mass spectrometry (LCMS/MS). Development of a high performance liquid chromatography (HPLC) method to analyse carbohydrates in beer. · The efficiency a pilot scale ultraviolet (UV-C) system at 254 nm to inactivate spoilage microorganisms spiked in commercial beer. Bacteria test were Lactobacillus brevis, Acetobacter pasteurianus and Saccharomyces cerevisiae · A pilot scale UV treatment of commercial and non-commercial lager beers at UV dosage of 1000 J/L. Following the UV treatment, the correlation between chemical analyses and sensory tests conducted by consumers’ tasters were investigated. · A pilot scale UV treatment of non-commercial beer brewed with reduced hops iso-α-acids (tetrahydro-iso-α-acids) at UV dosage of 1000 J/L. Sensory changes and chemical properties were investigated. · The development and optimisation of an UV light emitting diodes (UV-LED) bench scale apparatus. Chemical and microbiological tests were conducted to investigate the effect of UV-LEDs on beer at 250 nm and 275 nm wavelengths. | en_ZA |
dc.description.abstract | AFRIKAANSE OPSOMMING: Hierdie studie beskryf: · Die ontwikkeling van analitiese toerusting om die invloed van lig op die smaakontwikkeling in bier te bestudeer m.b.v gaschromatografie massa spektrometrie (GCMS) en vloeistofchromatografie massa spektrometrie/massa spektrometrie, asook die ontwikkeling van ‘n hoë druk vloeistofchromatografiese metode vir die analise van koolhidrate in bier. · Die doeltreffendheid van ‘n toetsskaal ultraviolet (UV-C) sisteem om die nadelige mikroorganismes waarmee die bier geïnnokuleer was, by 254 nm te inaktiveer.. Toetse is uitgevoer met die volgende bakterieë, Lactobacillus brevis, Acetobacter pasteuriants en Saccharomyces cerevisiae. · ‘n Toetsskaal UV behandeling van kommersiële en nie-kommersiële lager biere by ‘n UV dosering van 1000 J/L. Na UV behandeling is die verwantskap tussen chemiese analises en ‘n reeks sensoriese toetse deur vebruikers proeërs ondersoek.. · ‘n Toetsskaal UV behandeling van ‘n nie-kommersiële bier gebrou met verlaagde hops-iso-α-sure (tetrahidro-iso-α -sure) by UV dosering van 1000 J/L. Sensoriese veranderinge asook chemiese eienskappe is ondersoek. · Die ontwikkeling en optimalisering van ‘n UV-lig emissie diodes bankskaal apparaat. Chemiese en mikrobiologiese toetse is uitgevoer om die effek van UV lig op bier by 250 nm en 275 nm te ondersoek. | af_ZA |
dc.format.extent | xix, 353 p. : ill. | |
dc.identifier.uri | http://hdl.handle.net/10019.1/20365 | |
dc.language.iso | en_ZA | en_ZA |
dc.publisher | Stellenbosch : Stellenbosch University | en_ZA |
dc.rights.holder | Stellenbosch University | en_ZA |
dc.subject | Beer -- Microbiology | en_ZA |
dc.subject | Brewing -- Microbiology | en_ZA |
dc.subject | Liquid chromatography | en_ZA |
dc.subject | Gas chromatography | en_ZA |
dc.subject | Dissertations -- Biochemistry | en_ZA |
dc.subject | Theses -- Biochemistry | en_ZA |
dc.subject.other | Biochemistry | en_ZA |
dc.title | The effect of ultraviolet-C treatment on the biochemical composition of beer | en_ZA |
dc.type | Thesis | en_ZA |
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