Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

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dc.contributor.authorLiu, Zhuoen_ZA
dc.contributor.authorInokuma, Kentaroen_ZA
dc.contributor.authorHo, Shih‑Hsinen_ZA
dc.contributor.authorDen Haan, Riaanen_ZA
dc.contributor.authorHasunuma, Tomohisaen_ZA
dc.contributor.authorVan Zyl, Willem H.en_ZA
dc.contributor.authorKondo, Akihikoen_ZA
dc.date.accessioned2016-12-21T08:44:30Z
dc.date.available2016-12-21T08:44:30Z
dc.date.issued2015-09-26
dc.descriptionCITATION: Liu, Z. et al. 2015. Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae. Biotechnology for Biofuels, 8:162, doi:10.1186/s13068-015-0344-6.
dc.descriptionThe original publication is available at http://biotechnologyforbiofuels.biomedcentral.com
dc.description.abstractBackground: Engineering Saccharomyces cerevisiae to produce heterologous cellulases is considered as a promising strategy for production of bioethanol from lignocellulose. The production of cellulase is usually pursued by one of the two strategies: displaying enzyme on the cell surface or secreting enzyme into the medium. However, to our knowledge, the combination of the two strategies in a yeast strain has not been employed. Results: In this study, heterologous endoglucanase (EG) and cellobiohydrolase I (CBHI) were produced in a β-glucosidase displaying S. cerevisiae strain using cell-surface display, secretion, or a combined strategy. Strains EGD- CBHI-D and EG-S-CBHI-S (with both enzymes displayed on the cell surface or with both enzymes secreted to the surrounding medium) showed higher ethanol production (2.9 and 2.6 g/L from 10 g/L phosphoric acid swollen cellulose, respectively), than strains EG-D-CBHI-S and EG-S-CBHI-D (with EG displayed on cell surface and CBHI secreted, or vice versa). After 3-cycle repeated-batch fermentation, the cellulose degradation ability of strain EG-D-CBHI-D remained 60 % of the 1st batch, at a level that was 1.7-fold higher than that of strain EG-S-CBHI-S. Conclusions: This work demonstrated that placing EG and CBHI in the same space (on the cell surface or in the medium) was favorable for amorphous cellulose-based ethanol fermentation. In addition, the cellulolytic yeast strain that produced enzymes by the cell-surface display strategy performed better in cell-recycle batch fermentation compared to strains producing enzymes via the secretion strategy.en_ZA
dc.description.urihttp://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0344-6
dc.description.versionPublisher's version
dc.format.extent12 pages : illustrationsen_ZA
dc.identifier.citationLiu, Z. et al. 2015. Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae. Biotechnology for Biofuels, 8:162, doi:10.1186/s13068-015-0344-6.
dc.identifier.issn1754-6834 (online)
dc.identifier.otherdoi:10.1186/s13068-015-0344-6
dc.identifier.urihttp://hdl.handle.net/10019.1/100007
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Central
dc.rights.holderAuthors retain copyright
dc.subjectSecretionen_ZA
dc.subjectCellulaseen_ZA
dc.subjectEthanolen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.titleCombined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiaeen_ZA
dc.typeArticleen_ZA
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