Evaluation of a rapid screening test for rifampicin resistance in re-treatment tuberculosis patients in the Eastern Cape

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dc.contributor.authorAlbert, H.
dc.contributor.authorTrollip, A. P.
dc.contributor.authorSeaman, T.
dc.contributor.authorAbrahams, C.
dc.contributor.authorMole, R. J.
dc.contributor.authorJordaan, A.
dc.contributor.authorVictor, T.
dc.contributor.authorHoosain, E.
dc.date.accessioned2011-03-18T14:56:56Z
dc.date.available2011-03-18T14:56:56Z
dc.date.issued2007-09
dc.descriptionArticle
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.descriptionBibliography
dc.description.abstractBackground and objectives. Patients with multidrug-resistant (MDR) tuberculosis (TB) are at high risk of treatment failure. It is anticipated that early identification of MDR-TB and appropriate treatment will improve patient outcome and disease control. We evaluated the rapid detection of rifampicin resistance in previously treated TB patients, directly from acid-fast bacilli (AFB)-positive sputum using a phage-based test, FASTPlaque-Response (Biotec Laboratories Ltd, Ipswich, UK). The ability of rifampicin resistance to predict MDR-TB was also determined. Design. A prospective study was done comparing performance of the rapid phage test with conventional culture and drug susceptibility testing (DST) in AFB-positive TB patients. Setting. Five primary health clinics and one TB referral centre in the Port Elizabeth Metropolitan area, Eastern Cape. Outcome measures. Sensitivity, specificity and overall accuracy of the phage test were determined compared with gold standard culture and DST. Discrepant results were resolved by molecular detection of mutations conferring rifampicin resistance. The proportion of rifampicin-resistant strains that were MDR was also determined. Results. Previously treated patients were at a high risk of MDR-TB (35.7%). Sensitivity, specificity and overall accuracy of FASTPlaque-Response for rifampicin resistance determination were 95.4% (95% confidence interval (CI): 91.0-99.8%), 97.2% (95% CI: 94.5-99.9%) and 96.5% (95% CI: 94.1-98.9%) respectively compared with conventional DST (unresolved), calculated for specimens that had both FASTPlaque-Response and conventional DST results available. FASTPlaque-Response results were available in 2 days instead of 28-85 days with conventional DST. However, only 70.6% of FASTPlaque-Response results were interpretable compared with 86.3% of conventional DST results. The majority (95.5%) of rifampicin-resistant strains were MDR-TB. Conclusions. Rapid detection of rifampicin resistance using FASTPlaque-Response could contribute to improved management of patients at risk of MDR-TB, such as previously treated patients. However, improvement in control of specimen-related contamination is needed to ensure that a higher proportion of FASTPlaque-Response results are interpretable. Where indicated, early modification of therapy could improve patient prognosis and reduce disease transmission.
dc.description.versionPublishers' Version
dc.format.extentp. 858-863
dc.identifier.citationAlbert, H. et al. 2007. Evaluation of a rapid screening test for rifampicin resistance in re-treatment tuberculosis patients in the Eastern Cape. South African Medical Journal, 97(9), 858-863.
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7083
dc.publisherHealth and Medical Publishing Group (HMPG)
dc.rights.holderSAMJ reserves copyright of the material published
dc.subjectIsoniaziden_ZA
dc.subjectRifampicin resistance -- Testingen_ZA
dc.subjectAcid fast bacteriumen_ZA
dc.subjectAntibiotic sensitivityen_ZA
dc.subjectLung tuberculosis -- Treatmenten_ZA
dc.subjectMycobacterium tuberculosis -- Treatmenten_ZA
dc.subjectTuberculosis -- Patients -- Mortalityen_ZA
dc.titleEvaluation of a rapid screening test for rifampicin resistance in re-treatment tuberculosis patients in the Eastern Cape
dc.typeArticle
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