The effect of adenylate kinase on the glycolytic oscillations of Saccharomyces cerevisiae

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dc.contributor.advisorSnoep, Jacob Leenderten_ZA
dc.contributor.advisorVan Niekerk, Daviden_ZA
dc.contributor.advisorKouril, Jutta Theresaen_ZA
dc.contributor.authorVan Wyk, Morneen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.date.accessioned2019-02-22T08:42:03Z
dc.date.accessioned2019-04-17T08:04:01Z
dc.date.available2019-09-05T03:00:07Z
dc.date.issued2019-03
dc.descriptionThesis (MSc)--Stellenbosch University, 2019.en_ZA
dc.description.abstractENGLISH ABSTRACT: The regulation of phosphofructokinase (PFK) by ATP and AMP is a necessary feature for glycolytic oscillations. The enzyme adenylate kinase (AK) is responsible for the interconversion of ATP + AMP 2 ADP, and so influences the regulation of PFK. We present here, both experimentally and through model simulations, that a decrease in AK activity results in an increase in period time of the glycolytic oscillations. We show, for the rst time, that AK is a necessary component of these oscillations and an absence of AK activity results in no oscillations. Using an existing model for glycolytic oscillations, we incorperate a mechanistic equation for AK activity with parameters determined from ts to enzyme kinetic data. In the subsequent model simulations we piece apart the individual contributions of ATP and AMP towards these oscillations and find that a reduction in AK activity leads to changes in both ATP and AMP oscillation phase and amplitude. This causes changes in PFK T and R state switching, increasing the period time of the oscillations.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Die regulering van fosfofruktokinase (PFK) deur ATP en AMP is 'n noodsaaklike kenmerk vir glikolitiese ossillasies. Die ensiem adenylaatkinase (AK) is verantwoordelik vir die interkonversie van ATP + AMP 2 ADP, en beà nvloed ook die regulering van PFK. Ons bied hier, beide eksperimenteel en deur middel van simulasies, aan dat 'n afname in AK-aktiwiteit 'n toename in die tydperk van die glikolitiese ossillasies tot gevolg het. Ons wys vir die eerste keer dat AK 'n noodsaaklike komponent van hierdie ossillasies is en dat 'n afwesigheid van AK-aktiwiteit geen ossillasies tot gevolg het nie. Deur 'n bestaande model vir glikolitiese ossillasies te gebruik, inkorporeer ons 'n meganistiese vergelyking vir AK-aktiwiteit met parameters wat bepaal word uit pasings tot ensiemkinetiese data. In die daaropvolgende model simulasies onderskei ons die individuele bydraes van ATP en AMP na hierdie ossillasies en vind dat 'n vermindering in AK aktiwiteit lei tot veranderinge in beide ATPen AMP-ossillasiefase en amplitude. Dit veroorsaak veranderinge in PFK T en R staatskakelaars, wat die tydperkstyd van die ossillasies verhoog.af_ZA
dc.description.versionMastersen_ZA
dc.embargo.terms2019-09-05
dc.format.extentx, 78 leaves : illustrations (some color)
dc.identifier.urihttp://hdl.handle.net/10019.1/105593
dc.language.isoenen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch Universityen_ZA
dc.subjectAdenylase kinaseen_ZA
dc.subjectAdenosine triphosphateen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectOscillationsen_ZA
dc.subjectPhosphofructokinase 1en_ZA
dc.subjectUCTDen_ZA
dc.subjectAdenosine monophosphateen_ZA
dc.subjectGlycolsen_ZA
dc.titleThe effect of adenylate kinase on the glycolytic oscillations of Saccharomyces cerevisiaeen_ZA
dc.typeThesisen_ZA
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