An Evaluation of High‑Risk HPV in Squamous Cell Carcinomas of the Lip in a South African Cohort
| dc.contributor.author | Harbor, Sharon N. | en_ZA |
| dc.contributor.author | Schneider, Johann W. | en_ZA |
| dc.contributor.author | Solomons, Nadine | en_ZA |
| dc.contributor.author | Sanderson, Micheline | en_ZA |
| dc.contributor.author | Afrogheh, Amir H. | en_ZA |
| dc.date.accessioned | 2024-09-16T06:07:23Z | |
| dc.date.available | 2024-09-16T06:07:23Z | |
| dc.date.issued | 2024-05-06 | en_ZA |
| dc.description | The original publication is available at: https://link.springer.com | en_ZA |
| dc.description.abstract | Background To determine the prevalence of HR-HPV in a series of lip SCC from South African patients, using currently accepted HPV-testing methodologies and to define the clinical and histomorphologic features of HPV-associated lip SCC. Methods Fifty SCC of lip and 50 control cases were tested for HR-HPV using p16 and HR-HPV DNA PCR. p16-equivocal/positive and HPV DNA PCR-positive SCC were further evaluated for the expression of HPV-16 and HPV-18 mRNA transcripts using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to confirm transcriptionally active HPV. Results p16 was positive in 22% (n = 11) and equivocal in 4% (n = 2) of the SCC. One p16-positive case showed positivity for both HPV-16 DNA and HPV-16 E6/E7 mRNA transcripts (HPV prevalence rate of 2%). The HPV-positive case was non-keratinizing and occurred in an 80-year-old female. The two p16-equivocal cases were HR-HPV DNA positive and mRNA PCR negative. p16 was found to have a positive predictive value of 9%. Conclusion Findings from our cohort of lip SCC suggest that HR-HPV may have an insignificant role in the pathogenesis of SCC at this site. Due to its low ppv, p16 is insufficient to establish HR-HPV infection in SCC of the lip. The combination of p16 and DNA PCR appears to correlate with the presence of transcriptionally active virus. HPV E6/E7 mRNA detection is the gold standard for identifying HR-HPV. mRNA testing is not widely available in sub-Saharan Africa due to technical and financial constraints; however, the test appears to be of great value in p16-equivocal lip SCC. | en_ZA |
| dc.description.uri | https://link.springer.com/article/10.1007/s12105-024-01639-0 | en_ZA |
| dc.description.version | Publisher’s version | en_ZA |
| dc.format.extent | 8 pages | en_ZA |
| dc.identifier.citation | Harbor, S.N.,et al. An Evaluation of High-Risk HPV in Squamous Cell Carcinomas of the Lip in a South African Cohort. Head and Neck Pathol 18(36):8 pages . doi.10.1007/s12105-024-01639-0 | en_ZA |
| dc.identifier.doi | 10.1007/s12105-024-01639-0 | en_ZA |
| dc.identifier.issn | 1936-0568 (online) | en_ZA |
| dc.identifier.orcid | 0000-0001-5818-7945 | en_ZA |
| dc.identifier.uri | https://scholar.sun.ac.za/handle/10019.1/131133 | |
| dc.language.iso | en | |
| dc.publisher | Springer Nature | en_ZA |
| dc.rights.holder | Authors retain copyright | en_ZA |
| dc.title | An Evaluation of High‑Risk HPV in Squamous Cell Carcinomas of the Lip in a South African Cohort | en_ZA |
| dc.type | Article | en_ZA |