Highly active antiretroviral therapy alters sperm parameters and testicular antioxidant status in diet-induced obese rats
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Korean Society of Toxicology
Abstract
The efficacy of highly active antiretroviral therapy (HAART) has led to an increase demand for therapeutic use,
thereby necessitating investigation into drug toxicity. This study was designed to investigate the in vivo effects of
HAART on sperm parameters and testicular oxidative stress in lean and obese rats. Wistar rats (males, n = 40,
weighing 180~200 g) were assigned randomly into 4 groups and treated accordingly for 16 weeks as follows:
Control (C): lean group fed with standard rat chow; Diet induced obesity (DIO): obese animals fed a high caloric
diet; C + ART: lean animals treated with HAART; DIO + ART: obese animals treated with HAART. An antiretroviral
drug combination of Tenofovir, Emtricitabine and Efavirenz at a dose of 17, 26 and 50 mg/kg/day was
administered for the latter 6 weeks via jelly cube feeding. At the end of the experimental period, sperm analysis
was performed on sperm collected from the caudal epididymis, while the testis was homogenized for antioxidant
enzyme and lipid peroxidation assays. Results showed that HAART significantly decreased sperm motility
(p < 0.05) in both lean and obese animals, and viability (p < 0.05) in the DIO group. Testicular glutathione, catalase
and superoxide dismutase were significantly decreased (p < 0.05), while Thiobarbituric acid reactive substances
(TBARS) levels were significantly increased (p < 0.05) when the DIO+ART group was compared to
Control group. Thus, the decreased sperm qualities associated with HAART might be as a result of increased testicular
oxidative stress prominent in obese animals.
Description
CITATION: Oyeyipo, I. P., et al. 2018. Highly active antiretroviral therapy alters sperm parameters and testicular antioxidant status in diet-induced obese rats. Toxicological Research, 34(1):41-48, doi:10.5487/TR.2018.34.1.041.
The original publication is available at http://www.toxicolres.org
The original publication is available at http://www.toxicolres.org
Keywords
Highly active antiretroviral therapy -- Toxicity, Drugs -- Toxicity, Spermatozoa -- Effect of drugs on, Testis -- Effect of drugs on, Spermatozoa -- Physiology, Oxidative stress, Obesity
Citation
Oyeyipo, I. P., et al. 2018. Highly active antiretroviral therapy alters sperm parameters and testicular antioxidant status in diet-induced obese rats. Toxicological Research, 34(1):41-48, doi:10.5487/TR.2018.34.1.041