Prospective evaluation of host biomarkers other than interferon gamma in QuantiFERON Plus supernatants as candidates for the diagnosis of tuberculosis in symptomatic individuals

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dc.contributor.authorManngo, Portia M.en_ZA
dc.contributor.authorGutschmidt, Andreaen_ZA
dc.contributor.authorSnyders, Candice I.en_ZA
dc.contributor.authorMutavhatsindi, Hygonen_ZA
dc.contributor.authorManyelo, Charles M.en_ZA
dc.contributor.authorMakhoba, Nonjabulo S.en_ZA
dc.contributor.authorAhlers, Petrien_ZA
dc.contributor.authorHiemstra, Andrietteen_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorMcAnda, Shirleyen_ZA
dc.contributor.authorKidd, Martinen_ZA
dc.contributor.authorMalherbe, Stephanus T.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorChegou, Novel N.en_ZA
dc.date.accessioned2021-07-10T10:39:38Z
dc.date.available2021-07-10T10:39:38Z
dc.date.issued2019-07-15
dc.descriptionCITATION: Manngo, P. M. et al. 2019. Prospective evaluation of host biomarkers other than interferon gamma in QuantiFERON Plus supernatants as candidates for the diagnosis of tuberculosis in symptomatic individuals. Journal of Infection, 79(3):228-235, doi:10.1016/j.jinf.2019.07.007.
dc.descriptionThe original publication is available at https://www.journalofinfection.com
dc.description.abstractBackground: There is an urgent need for new tools for the diagnosis of TB. We evaluated the usefulness recently described host biomarkers in supernatants from the newest generation of the QuantiFERON test (QuantiFERON Plus) as tools for the diagnosis of active TB. Methods: We recruited individuals presenting at primary health care clinics in Cape Town, South Africa with symptoms requiring investigation for TB disease, prior to the establishment of a clinical diagnosis. Participants were later classified as TB or other respiratory diseases (ORD) based on the results of clinical and laboratory tests. Using a multiplex platform, we evaluated the concentrations of 37 host biomarkers in QuantiFERON Plus supernatants from study participants as tools for the diagnosis of TB. Results: Out of 120 study participants, 35(29.2%) were diagnosed with active TB, 69(57.5%) with ORD whereas 16(13.3%) were excluded. 14(11.6%) of the study participants were HIV infected. Although individ- ual host markers showed potential as diagnostic candidates, the main finding of the study was the identi- fication of a six-marker biosignature in unstimulated supernatants (Apo-ACIII, CXCL1, CXCL9, CCL8, CCL-1, CD56) which diagnosed TB with sensitivity and specificity of 73.9%(95% CI; 51.6–87.8) and 87.6%(95% CI; 77.2–94.5), respectively, after leave-one-out cross validation. Combinations between TB-antigen specific biomarkers also showed potential (sensitivity of 77.3% and specificity of 69.2%, respectively), with multi- ple biomarkers being significantly different between TB patients, Quantiferon Plus Positive and Quantif- eron Plus negative individuals with ORD, regardless of HIV status. Conclusions: Biomarkers detected in QuantiFERON Plus supernatants may contribute to adjunctive diag- nosis of TB.en_ZA
dc.description.sponsorshipEDCTP , grant no: DRIA2014-311
dc.description.sponsorshipNational Research Foundation
dc.description.sponsorshipICIDR (grant no: 5U01IA115619)
dc.description.versionPublisher's version
dc.format.extent8 pagesen_ZA
dc.identifier.citationManngo, P. M. et al. 2019. Prospective evaluation of host biomarkers other than interferon gamma in QuantiFERON Plus supernatants as candidates for the diagnosis of tuberculosis in symptomatic individuals. Journal of Infection, 79(3):228-235, doi:10.1016/j.jinf.2019.07.007.
dc.identifier.issn0163-4453 (online)
dc.identifier.otherdoi:10.1016/j.jinf.2019.07.007
dc.identifier.urihttp://hdl.handle.net/10019.1/110692
dc.language.isoen_ZAen_ZA
dc.publisherElsevieren_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectInducing factor, Interferon-gammaen_ZA
dc.subjectBiomarkersen_ZA
dc.titleProspective evaluation of host biomarkers other than interferon gamma in QuantiFERON Plus supernatants as candidates for the diagnosis of tuberculosis in symptomatic individualsen_ZA
dc.typeArticleen_ZA
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