A comprehensive analysis in one run – in-depth conformation studies of protein–polymer chimeras by asymmetrical flow field-flow fractionation †

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dc.contributor.authorKaupbayeva, Bibifatimaen_ZA
dc.contributor.authorMurata, Hironobuen_ZA
dc.contributor.authorMatyjaszewski, Krzysztofen_ZA
dc.contributor.authorRussell, Alan J.en_ZA
dc.contributor.authorBoye, Susanneen_ZA
dc.contributor.authorLederer, Albenaen_ZA
dc.date.accessioned2023-05-11T06:57:27Zen_ZA
dc.date.available2023-05-11T06:57:27Zen_ZA
dc.date.issued2021-09en_ZA
dc.descriptionCITATION: Kaupbayeva, B. et al. 2021. A comprehensive analysis in one run – in-depth conformation studies of protein–polymer chimeras by asymmetrical flow field-flow fractionation. Chemical Science 12(2021):9 pages. doi.10.1039/D1SC03033Gen_ZA
dc.descriptionThe original publication is available at: rsc.orgen_ZA
dc.description.abstractPolymer-based protein engineering has enabled the synthesis of a variety of protein–polymer conjugates that are widely applicable in therapeutic, diagnostic and biotechnological industries. Accurate characterizations of physical–chemical properties, in particular, molar masses, sizes, composition and their dispersities are critical parameters that determine the functionality and conformation of protein–polymer conjugates and are important for creating reproducible manufacturing processes. Most of the current characterization techniques suffer from fundamental limitations and do not provide an accurate understanding of a sample's true nature. In this paper, we demonstrate the advantage of asymmetrical flow field-flow fractionation (AF4) coupled with multiple detectors for the characterization of a library of complex, zwitterionic and neutral protein–polymer conjugates. This method allows for determination of intrinsic physical properties of protein–polymer chimeras from a single, rapid measurement.en_ZA
dc.description.versionPublisher’s versionen_ZA
dc.format.extent9 pagesen_ZA
dc.identifier.citationKaupbayeva, B. et al. 2021. A comprehensive analysis in one run – in-depth conformation studies of protein–polymer chimeras by asymmetrical flow field-flow fractionation. Chemical Science 12(2021):9 pages. doi.10.1039/D1SC03033Gen_ZA
dc.identifier.issn2041-6539 (Online)en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/126895en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherRoyal Society of Chemistryen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectAsymmetricalen_ZA
dc.subjectProtein–polymer chimerasen_ZA
dc.subjectConformation studiesen_ZA
dc.subjectFractionationen_ZA
dc.titleA comprehensive analysis in one run – in-depth conformation studies of protein–polymer chimeras by asymmetrical flow field-flow fractionation †en_ZA
dc.typeArticleen_ZA
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