Molecular characterisation and epidemiology of enterovirus-associated aseptic meningitis in the Western and Eastern Cape Provinces, South Africa 2018–2019

Simple item page
dc.contributor.authorNkosi, Nokwazien_ZA
dc.contributor.authorPreiser, Wolfgangen_Za
dc.contributor.authorVan Zyl, Gerten_ZA
dc.contributor.authorClaassen, Mathildaen_ZA
dc.contributor.authorCronje, Nadineen_ZA
dc.contributor.authorMaritz, Jeanen_ZA
dc.contributor.authorNewman, Howarden_ZA
dc.contributor.authorMcCarthy, Kerriganen_ZA
dc.contributor.authorNtshoe, Genevieen_ZA
dc.contributor.authorEssel, Vivienen_ZA
dc.contributor.authorKorsman, Stephenen_ZA
dc.contributor.authorHardie, Dianaen_ZA
dc.contributor.authorSmuts, Heidien_ZA
dc.date.accessioned2023-03-24T12:10:31Zen_ZA
dc.date.available2023-03-24T12:10:31Zen_ZA
dc.date.issued2021-04en_ZA
dc.descriptionCITATION: Nkosi, N. et al. 2021. Molecular characterisation and epidemiology of enterovirus-associated aseptic meningitis in the Western and Eastern Cape Provinces, South Africa 2018–2019. Journal of Clinical Virology 139(2021):6 pages. doi.10.1016/j.jcv.2021.104845en_ZA
dc.descriptionThe original publication is available at: homepage: .elsevier.comen_ZA
dc.description.abstractBackground: Enteroviruses are amongst the most common causes of aseptic meningitis. Between November 2018 and May 2019, an outbreak of enterovirus-associated aseptic meningitis cases was noted in the Western and Eastern Cape Provinces, South Africa. Objectives: To describe the epidemiology and phylogeography of enterovirus infections during an aseptic meningitis outbreak in the Western and Eastern Cape Provinces of South Africa. Methods: Cerebrospinal fluid samples from suspected cases were screened using a polymerase chain reaction targeting the 5’UTR. Confirmed enterovirus-associated meningitis samples underwent molecular typing through species–specific VP1/VP2 primers and pan-species VP1 primers. Results: Between November 2018 and May 2019, 3497 suspected cases of aseptic meningitis were documented in the Western and Eastern Cape Provinces. Median age was 8 years (range 0–61), interquartile range (IQR=4–13 years), 405/735 (55%) male. 742/3497 (21%) cases were laboratory – confirmed enterovirus positive by routine diagnostic PCR targeting the 5’UTR. 128/742 (17%) underwent molecular typing by VP1 gene sequencing. Echovirus 4 (E4) was detected in 102/128 (80%) cases. Echovirus 9 was found in 7%, Coxsackievirus A13 in 3%. 10 genotypes contributed to the remaining 10% of cases. Synonymous mutations were found in most cases, with sporadic amino acid changes in 13 (12.7%) cases. Conclusion: The aseptic meningitis outbreak was associated with echovirus 4. Stool samples are valuable for molecular typing in CSF confirmed EV-associated aseptic meningitis.en_ZA
dc.description.versionPublisher’s versionen_ZA
dc.format.extent6 pagesen_ZA
dc.identifier.citationNkosi, N. et al. 2021. Molecular characterisation and epidemiology of enterovirus-associated aseptic meningitis in the Western and Eastern Cape Provinces, South Africa 2018–2019. Journal of Clinical Virology 139(2021):6 pages. doi.10.1016/j.jcv.2021.104845en_ZA
dc.identifier.otherdoi.10.1016/j.jcv.2021.104845en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/126711en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherElsevier B.V.en_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectEnteroviruses -- Pathogenicity -- South Africaen_ZA
dc.subjectMeningitis -- South Africaen_ZA
dc.subjectMolecular epidemiology -- South Africaen_ZA
dc.subjectECHO viruses -- South Africaen_ZA
dc.titleMolecular characterisation and epidemiology of enterovirus-associated aseptic meningitis in the Western and Eastern Cape Provinces, South Africa 2018–2019en_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nokwazi_molecular_2021.pdf
Size:
1.64 MB
Format:
Adobe Portable Document Format
Description:
download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Medical Virology)