Effect of chronic desipramine treatment on adrenoceptor modulation of [3H]dopamine release from rat nucleus accumbens slices

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dc.contributor.authorNurse B.
dc.contributor.authorRussell V.A.
dc.contributor.authorTaljaard J.J.F.
dc.date.accessioned2011-05-15T16:15:45Z
dc.date.available2011-05-15T16:15:45Z
dc.date.issued1985
dc.description.abstractThe effects of α2- and β-adrenoceptor agonists on the 25 mM K+-induced release of [3H]dopamine ([3H]DA) from nucleus accumbens slices of chronic desipramine (DMI)- and saline-treated rats were investigated using a superfusion technique. The K+-induced release of [3H]DA from nucleus accumbens slices was shown to be Ca2+ dependent and to be enhanced by ascorbic acid. In experiments with isoproterenol, ascorbic acid was added to the superfusion media in order to prevent the otherwise rapid oxidation of the drug. The K+-induced release of [3H]DA from nucleus accumbens slices of saline-treated rats was significantly decreased by the α2-adrenoceptor agonist, clonidine (10 μM; 89 ± 2.4% of control values; P < 0.002), and significantly enhanced by the β-adrenoceptor agonist, isoproterenol (1 and 10 μM; 122 ± 4.3 and 171 ± 2.9% of control values, P < 0.002 and P < 0.001, respectively). The basal release of [3H]DA was strongly enhanced by 10 μM but not 1 μM isoproterenol. Chronic DMI pretreatment (10 mg/kg i.p. for 28 days) did not significantly alter the K+-induced release of [3H]DA. Chronic DMI treatment attenuated the α2-adrenoceptor-mediated inhibition of [3H]DA release, while the β-adrenoceptor-mediated stimulation remained unchanged. The net effect of chronic DMI treatment therefore would appear to be a facilitation of dopaminergic neurotransmission in the mesolimbic system. This is consistent with behavioural evidence which suggests that the function of the mesolimbic dopaminergic reward system is facilitated by chronic treatment with antidepressant drugs.
dc.description.versionArticle
dc.identifier.citationBrain Research
dc.identifier.citation334
dc.identifier.citation2
dc.identifier.issn00068993
dc.identifier.other10.1016/0006-8993(85)90215-X
dc.identifier.urihttp://hdl.handle.net/10019.1/13470
dc.subjectadrenergic receptor
dc.subjectalpha 2 adrenergic receptor
dc.subjectascorbic acid
dc.subjectbeta adrenergic receptor
dc.subjectclonidine
dc.subjectdesipramine
dc.subjectisoprenaline
dc.subjectnialamide
dc.subjectradioisotope
dc.subjectanimal experiment
dc.subjectcentral nervous system
dc.subjectdopamine h 3
dc.subjectdrug interaction
dc.subjecteighth nerve nucleus
dc.subjectnerve cell membrane potential
dc.subjectnervous system
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrat
dc.subjectAnimal
dc.subjectCalcium
dc.subjectClonidine
dc.subjectComparative Study
dc.subjectDesipramine
dc.subjectDopamine
dc.subjectDrug Interactions
dc.subjectIn Vitro
dc.subjectIsoproterenol
dc.subjectMale
dc.subjectNucleus Accumbens
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectReceptors, Adrenergic
dc.subjectSeptal Nuclei
dc.subjectSupport, Non-U.S. Gov't
dc.titleEffect of chronic desipramine treatment on adrenoceptor modulation of [3H]dopamine release from rat nucleus accumbens slices
dc.typeArticle
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