Zona pellucida mediated acrosome reaction and sperm morphology

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dc.contributor.authorFranken D.R.
dc.contributor.authorBastiaan H.S.
dc.contributor.authorKidson A.
dc.contributor.authorWranz P.
dc.contributor.authorHabenicht U.-F.
dc.date.accessioned2011-05-15T16:15:27Z
dc.date.available2011-05-15T16:15:27Z
dc.date.issued1997
dc.description.abstractSperm samples from 29 men randomly selected from the andrology laboratory, were used to evaluate acrosome reaction response to solubilized human zona pellucida. Capacitated sperm samples were exposed to a solution containing 2 zona pellucidae (ZP) per μl for 60 min, after which acrosomal status were recorded using a PSA-FITC technique. Controls included samples supplied by fertile sperm donors. After completion of acrosome reaction studies, patient samples were divided according to the percentage of morphologically normal spermatozoa. Three basic groups were identified, namely, fertile donors, teratozoospermic (normal sperm morphology 5-14%; n= 25) and severely teratozoospermic (normal sperm morphology <4%; n=4) groups. The mean percent normal sperm were 15.8±0.9, 10.4±0.7 and 2.7±0.7, respectively, for normozoospermic donors, teratozoospermic and severely teratozoospermic men. The mean percentage (±SE) ZP mediated acrosome reacted sperm among teratozoospermic and severely teratozoospermic cases was 25.8% ± 0.9 and 19.0% ± 0.9 (P= 0.001), compared to 36.8%±0.9 for the donor controls. Results were analysed and expressed as correlations between sperm morphology and acrosomal response to human solubilized zona pellucida, spontaneous and calcium ionophore induced acrosome reaction. Predictive values for acrosome responsiveness were depicted with ROC curve analyses. Sperm morphology evaluated by strict criteria correlated positively and highly significantly with the responsiveness of the acrosome reaction (r=0.91, P=0.0001). At a morphology cut-off value of 4%, the ROC curve analysis showed sperm morphology to be highly predictive of zona pellucida induced acrosome responsiveness with a sensitivity of 100% and negative predictive value of 100%. Spontaneous and calcium ionophore induced acrosome reactions revealed no correlation with sperm morphology. It was concluded that (i) morphological features of human spermatozoa are indicative of specific functional characteristics; (ii) zona pellucida induction of the acrosome reaction is superior, as a predictor of sperm morphology, compared to calcium ionophore induced and spontaneous acrosome reactions.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation29
dc.identifier.citation6
dc.identifier.issn03034569
dc.identifier.urihttp://hdl.handle.net/10019.1/13342
dc.subjectcalcium ionophore
dc.subjectacrosome reaction
dc.subjectarticle
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectdiagnostic value
dc.subjecthuman
dc.subjecthuman cell
dc.subjectinfertility therapy
dc.subjectmale
dc.subjectmale infertility
dc.subjectreceiver operating characteristic
dc.subjectsemen analysis
dc.subjectspermatozoon capacitation
dc.subjectspermatozoon motility
dc.subjectzona pellucida
dc.subjectAcrosome
dc.subjectCalcimycin
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectSperm-Ovum Interactions
dc.subjectSpermatozoa
dc.subjectZona Pellucida
dc.titleZona pellucida mediated acrosome reaction and sperm morphology
dc.typeArticle
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