A comparative analysis of grade 1 and grade 2 water in the Tygerberg Hospital in vitro fertilisation programme
Date
1987-02
Authors
Kruger, T. F.
Van Wyk, H.
Stander, F. S. H.
Smith, K.
Menkveld, R.
Van der Merwe, J. P.
Journal Title
Journal ISSN
Volume Title
Publisher
Health and Medical Publishing Group (HMPG)
Abstract
There are conflicting reports in the literature about the effect of the water source for growth and insemination media in an in vitro fertilisation (IVF) programme. A controlled trial was carried out in which two-cell mouse embryos were cultured in Ham F10 medium containing either grade 1 water (test group) or grade 2 water (control group). Of the two-cell embryos, 92,0% (69 of 75) in the test group and 91,8% (67 of 73) in the control group cleaved to the blastocyst stage (no statistically significant difference). On the basis of this experiment grade 2 water was used in the Tygerberg Hospital human IVF programme with good results. A continuing pregnancy rate of 23% per embryo transfer over a 1-year period is reported.
Description
The original publication is available at http://www.samj.org.za
Keywords
In vitro fertilisation programme
Citation
Kruger, T. F., Van Wyk [et al] 1987. A comparative analysis of grade 1 and grade 2 water in the Tygerberg Hospital in vitro fertilisation programme. South African Medical Journal, 71, 162-163.