Hanseniaspora uvarum from winemaking environments show spatial and temporal genetic clustering

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dc.contributor.authorAlbertin, Warrenen_ZA
dc.contributor.authorSetati, Mathabatha E.en_ZA
dc.contributor.authorMiot-Sertier, Cecileen_ZA
dc.contributor.authorMostert, Talitha T.en_ZA
dc.contributor.authorColonna-Ceccaldi, Benoiten_ZA
dc.contributor.authorCoulon, Joanaen_ZA
dc.contributor.authorGirard, Patricken_ZA
dc.contributor.authorMoine, Virginieen_ZA
dc.contributor.authorPillet, Myriamen_ZA
dc.contributor.authorSalin, Francken_ZA
dc.contributor.authorBely, Marinaen_ZA
dc.contributor.authorDivol, Benoiten_ZA
dc.contributor.authorMasneuf-Pomarede, Isabelleen_ZA
dc.date.accessioned2017-01-30T08:06:48Z
dc.date.available2017-01-30T08:06:48Z
dc.date.issued2016-01-20
dc.descriptionCITATION: Albertin, W. et al. 2016. Hanseniaspora uvarum from winemaking environments show spatial and temporal genetic clustering. Frontiers in Microbiology, 6:1569, doi:10.3389/fmicb.2015.01569.
dc.descriptionThe original publication is available at http://journal.frontiersin.org/journal/microbiology
dc.description.abstractHanseniaspora uvarum is one of the most abundant yeast species found on grapes and in grape must, at least before the onset of alcoholic fermentation (AF) which is usually performed by Saccharomyces species. The aim of this study was to characterize the genetic and phenotypic variability within the H. uvarum species. One hundred and fifteen strains isolated from winemaking environments in different geographical origins were analyzed using 11 microsatellite markers and a subset of 47 strains were analyzed by AFLP. H. uvarum isolates clustered mainly on the basis of their geographical localization as revealed by microsatellites. In addition, a strong clustering based on year of isolation was evidenced, indicating that the genetic diversity of H. uvarum isolates was related to both spatial and temporal variations. Conversely, clustering analysis based on AFLP data provided a different picture with groups showing no particular characteristics, but provided higher strain discrimination. This result indicated that AFLP approaches are inadequate to establish the genetic relationship between individuals, but allowed good strain discrimination. At the phenotypic level, several extracellular enzymatic activities of enological relevance (pectinase, chitinase, protease, β-glucosidase) were measured but showed low diversity. The impact of environmental factors of enological interest (temperature, anaerobia, and copper addition) on growth was also assessed and showed poor variation. Altogether, this work provided both new analytical tool (microsatellites) and new insights into the genetic and phenotypic diversity of H. uvarum, a yeast species that has previously been identified as a potential candidate for co-inoculation in grape must, but whose intraspecific variability had never been fully assessed.en_ZA
dc.description.urihttp://journal.frontiersin.org/article/10.3389/fmicb.2015.01569/full
dc.description.versionPublisher's version
dc.format.extent16 pages
dc.identifier.citationAlbertin, W. et al. 2016. Hanseniaspora uvarum from winemaking environments show spatial and temporal genetic clustering. Frontiers in Microbiology, 6:1569, doi:10.3389/fmicb.2015.01569.
dc.identifier.issn1664-302X (online)
dc.identifier.otherdoi:10.3389/fmicb.2015.01569
dc.identifier.urihttp://hdl.handle.net/10019.1/100554
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers
dc.rights.holderAuthors retain copyright
dc.subjectHanseniaspora uvarumen_ZA
dc.subjectWine and winemaking -- Bacteriologyen_ZA
dc.subjectIntraspecific diversityen_ZA
dc.subjectMicrosatellitesen_ZA
dc.subjectPhenotypic screeningen_ZA
dc.subjectFermentationen_ZA
dc.subjectYeast fungi -- Biotechnologyen_ZA
dc.titleHanseniaspora uvarum from winemaking environments show spatial and temporal genetic clusteringen_ZA
dc.typeArticleen_ZA
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