Comparison between propidium iodide and 7-amino-actinomycin-D for viability assessment during flow cytometric analyses of the human sperm acrosome

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dc.contributor.authorFalzone N.
dc.contributor.authorHuyser C.
dc.contributor.authorFranken D.R.
dc.contributor.authorFalzone N.
dc.contributor.authorHuyser C.
dc.contributor.authorFranken D.R.
dc.date.accessioned2011-05-15T16:15:24Z
dc.date.accessioned2011-05-15T16:15:24Z
dc.date.available2011-05-15T16:15:24Z
dc.date.available2011-05-15T16:15:24Z
dc.date.issued2010
dc.date.issued2010
dc.description.abstractEvaluation of the acrosome reaction can shed light on the fertilising competence of spermatozoa. To eliminate false-positive results when evaluating the acrosome status of human sperm cells, two viability probes propidium iodide (PI) and 7-amino-actinomycin D (7-AAD) were compared for their ability to stain nonviable cells post-fixation and permeabilisation. Both the mean fluorescence and % dead cells differed significantly with time (P < 0.0001). Unlike PI, 7-AAD did not leach from cells and fluorescence remained stable for up to 4 h. Furthermore, 7-AAD proved to be a proficient marker to exclude dead sperm cells during flow cytometric evaluation of ionophore-induced acrosome reaction. © 2009 Blackwell Publishing Ltd.
dc.description.abstractEvaluation of the acrosome reaction can shed light on the fertilising competence of spermatozoa. To eliminate false-positive results when evaluating the acrosome status of human sperm cells, two viability probes propidium iodide (PI) and 7-amino-actinomycin D (7-AAD) were compared for their ability to stain nonviable cells post-fixation and permeabilisation. Both the mean fluorescence and % dead cells differed significantly with time (P < 0.0001). Unlike PI, 7-AAD did not leach from cells and fluorescence remained stable for up to 4 h. Furthermore, 7-AAD proved to be a proficient marker to exclude dead sperm cells during flow cytometric evaluation of ionophore-induced acrosome reaction. © 2009 Blackwell Publishing Ltd.
dc.description.versionArticle
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation42
dc.identifier.citation1
dc.identifier.citationAndrologia
dc.identifier.citation42
dc.identifier.citation1
dc.identifier.issn03034569
dc.identifier.issn03034569
dc.identifier.other10.1111/j.1439-0272.2009.00949.x
dc.identifier.other10.1111/j.1439-0272.2009.00949.x
dc.identifier.urihttp://hdl.handle.net/10019.1/13322
dc.identifier.urihttp://hdl.handle.net/10019.1/13322
dc.subjectionophore; propidium iodide; acrosome; acrosome reaction; adult; article; cell membrane permeability; clinical assessment; comparative study; controlled study; flow cytometry; fluorescence; human; human cell; male; staining; time; Acrosome; Acrosome Reaction; Adult; Dactinomycin; False Positive Reactions; Flow Cytometry; Fluorescent Dyes; Humans; Male; Microscopy, Fluorescence; Propidium; Spermatozoa
dc.subjectionophore
dc.subjectpropidium iodide
dc.subjectacrosome
dc.subjectacrosome reaction
dc.subjectadult
dc.subjectarticle
dc.subjectcell membrane permeability
dc.subjectclinical assessment
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectflow cytometry
dc.subjectfluorescence
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectstaining
dc.subjecttime
dc.subjectAcrosome
dc.subjectAcrosome Reaction
dc.subjectAdult
dc.subjectDactinomycin
dc.subjectFalse Positive Reactions
dc.subjectFlow Cytometry
dc.subjectFluorescent Dyes
dc.subjectHumans
dc.subjectMale
dc.subjectMicroscopy, Fluorescence
dc.subjectPropidium
dc.subjectSpermatozoa
dc.titleComparison between propidium iodide and 7-amino-actinomycin-D for viability assessment during flow cytometric analyses of the human sperm acrosome
dc.titleComparison between propidium iodide and 7-amino-actinomycin-D for viability assessment during flow cytometric analyses of the human sperm acrosome
dc.typeArticle
dc.typeArticle
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