Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss physcomitrella patens

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dc.contributor.authorMdodana, Ntombizanele T.en_ZA
dc.contributor.authorJewell, Jonathan F.en_ZA
dc.contributor.authorPhiri, Ethel E.en_ZA
dc.contributor.authorSmith, Marthinus L.en_ZA
dc.contributor.authorOberlander, Kennethen_ZA
dc.contributor.authorMahmoodi, Saireen_ZA
dc.contributor.authorKossmann, Jensen_ZA
dc.contributor.authorLloyd, James R.en_ZA
dc.date.accessioned2019-10-23T06:41:21Z
dc.date.available2019-10-23T06:41:21Z
dc.date.issued2019
dc.descriptionCITATION: Mdodana, N. T., et al. 2019. Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss physcomitrella patens. Scientific Reports, 9:15114, doi:10.1038/s41598-019-51632-9 1.en_ZA
dc.descriptionThe original publication is available at https://www.nature.comen_ZA
dc.description.abstractThe role of starch degradation in non-vascular plants is poorly understood. To expand our knowledge of this area, we have studied this process in Physcomitrella patens. This has been achieved through examination of the step known to initiate starch degradation in angiosperms, glucan phosphorylation, catalysed by glucan, water dikinase (GWD) enzymes. Phylogenetic analysis indicates that GWD isoforms can be divided into two clades, one of which contains GWD1/GWD2 and the other GWD3 isoforms. These clades split at a very early stage within plant evolution, as distinct sequences that cluster within each were identified in all major plant lineages. Of the five genes we identified within the Physcomitrella genome that encode GWD-like enzymes, two group within the GWD1/GWD2 clade and the others within the GWD3 clade. Proteins encoded by both loci in the GWD1/GWD2 clade, named PpGWDa and PpGWDb, are localised in plastids. Mutations of either PpGWDa or PpGWDb reduce starch phosphate abundance, however, a mutation at the PpGWDa locus had a much greater influence than one at PpGWDb. Only mutations affecting PpGWDa inhibited starch degradation. Mutants lacking this enzyme also failed to develop gametophores, a phenotype that could be chemically complemented using glucose supplementation within the growth medium.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-019-51632-9
dc.description.versionPublisher's versionen_ZA
dc.format.extent13 pages : illustrationsen_ZA
dc.identifier.citationMdodana, N. T., et al. 2019. Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss physcomitrella patens. Scientific Reports, 9:15114, doi:10.1038/s41598-019-51632-9 1en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-019-51632-9 1
dc.identifier.urihttp://hdl.handle.net/10019.1/106701
dc.language.isoen_ZAen_ZA
dc.publisherNature Researchen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectPhyscomitrella patensen_ZA
dc.subjectStarch degradationen_ZA
dc.subjectGlucan, water dikinase enzymesen_ZA
dc.subjectPlant molecular physiologyen_ZA
dc.titleMutations in glucan, water dikinase affect starch degradation and gametophore development in the moss physcomitrella patensen_ZA
dc.typeArticleen_ZA
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