HHV-8 subtypes in South Africa: Identification of a case suggesting a novel B variant

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dc.contributor.authorTreurnicht F.K.
dc.contributor.authorEngelbrecht S.
dc.contributor.authorTaylor M.B.
dc.contributor.authorSchneider J.W.
dc.contributor.authorVan Rensburg E.J.
dc.date.accessioned2011-05-15T16:17:03Z
dc.date.available2011-05-15T16:17:03Z
dc.date.issued2002
dc.description.abstractHuman herpesvirus 8 (HHV-8) has been identified as the causative agent for all forms of Kaposi's sarcoma and is also associated with the development of body cavity- based B-cell lymphomas and multicentric Castleman's disease. HHV-8 genomes are now classified into five major subtypes (A-E) that reflect sequence heterogeneity in the highly variable open reading frame (ORF) K1. To identify HHV-8 subtypes associated with different forms of Kaposi's sarcoma, we compared the ORF 26 and ORF-K1 gene sequences from South African patients with the prototype strains of the major subtypes, as well as published sequences from other African strains. DNA prepared from Kaposi's sarcoma biopsies and/or peripheral blood lymphocytes were available from 14 patients with postrenal transplant (iatrogenic) Kaposi's sarcoma, six patients with the African endemic form, and one patient with AIDS-related body cavity-based B-cell lymphoma. We identified a B2 subtype in six patients, four of whom also had a novel B5 type ORF 26 polymorphism. Two patients had B2 type patterns for both the ORF 26 and ORF-K1 genes. The ORF-K1 subtype A5 was identified in samples from three patients with a B3/C2 type polymorphism in the ORF 26 gene. A novel ORF-K1 B variant strain was identified in a patient with African endemic Kaposi's sarcoma, who also had a B3/C2 class ORF 26 pattern. In 58.3% of iatrogenic Kaposi's sarcoma patients, a B5-type ORF 26 gene sequence pattern was identified. No association was found among particular subtypes, geographical origin of patients, or clinical presentation. © 2002 Wiley-Liss, Inc.
dc.description.versionArticle
dc.identifier.citationJournal of Medical Virology
dc.identifier.citation66
dc.identifier.citation2
dc.identifier.issn01466615
dc.identifier.other10.1002/jmv.2135
dc.identifier.urihttp://hdl.handle.net/10019.1/14050
dc.subjectadult
dc.subjectaged
dc.subjectangiofollicular lymph node hyperplasia
dc.subjectarticle
dc.subjectB cell lymphoma
dc.subjectclinical article
dc.subjectDNA polymorphism
dc.subjectendemic disease
dc.subjectfemale
dc.subjecthuman
dc.subjectHuman herpesvirus 8
dc.subjectKaposi sarcoma
dc.subjectmale
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectopen reading frame
dc.subjectsequence analysis
dc.subjectSouth Africa
dc.subjectstrain difference
dc.subjectvirus characterization
dc.subjectvirus genome
dc.subjectAdult
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectAmino Acid Sequence
dc.subjectDNA, Viral
dc.subjectFemale
dc.subjectHerpesvirus 8, Human
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMolecular Sequence Data
dc.subjectOpen Reading Frames
dc.subjectPhylogeny
dc.subjectSarcoma, Kaposi
dc.subjectSequence Analysis, DNA
dc.subjectSouth Africa
dc.subjectVariation (Genetics)
dc.subjectViral Proteins
dc.subjectHerpesviridae
dc.subjectHuman herpesvirus 8
dc.titleHHV-8 subtypes in South Africa: Identification of a case suggesting a novel B variant
dc.typeArticle
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