Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants

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dc.contributor.authorEmani, C. Saoen_ZA
dc.contributor.authorWilliams, M. J.en_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorTaylor, M. J. C.en_ZA
dc.contributor.authorCarolis, C.en_ZA
dc.contributor.authorWiid, I. J.en_ZA
dc.contributor.authorBaker, B.en_ZA
dc.date.accessioned2020-02-05T12:45:48Z
dc.date.available2020-02-05T12:45:48Z
dc.date.issued2018
dc.descriptionCITATION: Emani, C. S., et al. 2018. Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants. Scientific Data, 5:180184, doi:10.1038/sdata.2018.184.
dc.descriptionThe original publication is available at https://www.nature.com
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund
dc.description.abstractMycothiol (MSH) and ergothioneine (ERG) are thiols able to compensate for each other to protect mycobacteria against oxidative stress. Gamma-glutamylcysteine (GGC), another thiol and an intermediate in ERG biosynthesis has detoxification abilities. Five enzymes are involved in ERG biosynthesis, namely EgtA, EgtB, EgtC, EgtD and EgtE. The role of these enzymes in the production of ERG had been unclear. On the other hand, the enzyme MshA is known to be essential for MSH biosynthesis. In this manuscript, we describe the raw data of the generation and characterization of Mycobacterium tuberculosis (M.tb) mutants harbouring a deletion of the gene coding for each of these enzymes, and the raw data of the phenotypic characterization of the obtained thiol-deficient M.tb mutants. High throughput screening (HTS) of off-patent drugs and natural compounds revealed few compounds that displayed a higher activity against the thiol-deficient mutants relative to the wild-type strain. The mode of action of these drugs was further investigated. Raw data displaying these results are described here.en_ZA
dc.description.urihttps://www.nature.com/articles/sdata2018184
dc.description.versionPublisher's version
dc.format.extent14 pages
dc.identifier.citationEmani, C. S., et al. 2018. Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants. Scientific Data, 5:180184, doi:10.1038/sdata.2018.184
dc.identifier.issn2052-4463 (online)
dc.identifier.otherdoi:10.1038/sdata.2018.184
dc.identifier.urihttp://hdl.handle.net/10019.1/107439
dc.language.isoen_ZAen_ZA
dc.publisherNature Research
dc.rights.holderAuthors retain copyright
dc.subjectMycobacterium tuberculosis -- Analysisen_ZA
dc.subjectThiols -- Mechanism of actionen_ZA
dc.subjectMycobacteria -- Mutationen_ZA
dc.subjectEnzymes -- Synthesisen_ZA
dc.subjectOxidative stressen_ZA
dc.subjectGenotype-environment interactionen_ZA
dc.subjectPhenotype -- Geneticsen_ZA
dc.subjectHigh throughput screening (Drug development) -- Methodologyen_ZA
dc.titleGeneration and characterization of thiol-deficient Mycobacterium tuberculosis mutantsen_ZA
dc.typeArticleen_ZA
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