Complete nucleotide sequence of a South African isolate of grapevine fanleaf virus and its associated satellite RNA

Simple item page
dc.contributor.authorLamprecht, Renate L.
dc.contributor.authorSpaltman, Monique
dc.contributor.authorStephan, Dirk
dc.contributor.authorWetzel, Thierry
dc.contributor.authorBurger, Johan T.
dc.date.accessioned2014-02-13T14:23:22Z
dc.date.available2014-02-13T14:23:22Z
dc.date.issued2013-07
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at http://www.mdpi.com/journal/virusesen_ZA
dc.description.abstractThe complete sequences of RNA1, RNA2 and satellite RNA have been determined for a South African isolate of Grapevine fanleaf virus (GFLV-SACH44). The two RNAs of GFLV-SACH44 are 7,341 nucleotides (nt) and 3,816 nt in length, respectively, and its satellite RNA (satRNA) is 1,104 nt in length, all excluding the poly(A) tail. Multiple sequence alignment of these sequences showed that GFLV-SACH44 RNA1 and RNA2 were the closest to the South African isolate, GFLV-SAPCS3 (98.2% and 98.6% nt identity, respectively), followed by the French isolate, GFLV-F13 (87.3% and 90.1% nt identity, respectively). Interestingly, the GFLV-SACH44 satRNA is more similar to three Arabis mosaic virus satRNAs (85%–87.4% nt identity) than to the satRNA of GFLV-F13 (81.8% nt identity) and was most distantly related to the satRNA of GFLV-R2 (71.0% nt identity). Full-length infectious clones of GFLV-SACH44 satRNA were constructed. The infectivity of the clones was tested with three nepovirus isolates, GFLV-NW, Arabis mosaic virus (ArMV)-NW and GFLV-SAPCS3. The clones were mechanically inoculated in Chenopodium quinoa and were infectious when co-inoculated with the two GFLV helper viruses, but not when co-inoculated with ArMV-NW.en_ZA
dc.description.sponsorshipStellenbosch Universityen_ZA
dc.description.versionPublishers' versionen_ZA
dc.format.extentp. 1815-1823 : ill.
dc.identifier.citationLamprecht, R. L. et al. 2013. Complete nucleotide sequence of a South African isolate of grapevine fanleaf virus and its associated satellite RNA. Viruses, 5(7):1815-1823, doi:10.3390/v5071815.en_ZA
dc.identifier.issn1999-4915 (online)
dc.identifier.otherdoi:10.3390/v5071815
dc.identifier.urihttp://hdl.handle.net/10019.1/86148
dc.language.isoen_ZAen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectGrapevine fanleaf virus -- Geneticsen_ZA
dc.subjectArabis mosaic virusen_ZA
dc.subjectPhylogeneticsen_ZA
dc.titleComplete nucleotide sequence of a South African isolate of grapevine fanleaf virus and its associated satellite RNAen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
lamprecht_complete_2013.pdf
Size:
475.57 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.95 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Genetics)