A sensitive urinary lipoarabinomannan test for tuberculosis

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dc.contributor.authorHamasur, Bestonen_ZA
dc.contributor.authorBruchfeld, Judithen_ZA
dc.contributor.authorVan Helden, Paulen_ZA
dc.contributor.authorKallenius, Gunillaen_ZA
dc.contributor.authorSvenson, Stefanen_ZA
dc.date.accessioned2016-08-23T08:51:51Z
dc.date.available2016-08-23T08:51:51Z
dc.date.issued2015
dc.descriptionCITATION: Hamasur, B., et al. 2015. A sensitive urinary lipoarabinomannan test for tuberculosis. PLoS ONE, 10(4):1-11, doi:10.1371/journal.pone.0123457.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractWe have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIVnegative TB patients was very low.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0123457
dc.description.versionPublisher's version
dc.format.extent11 pages
dc.identifier.citationHamasur, B., et al. 2015. A sensitive urinary lipoarabinomannan test for tuberculosis. PLoS ONE, 10(4):1-11, doi:10.1371/journal.pone.0123457
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0123457
dc.identifier.urihttp://hdl.handle.net/10019.1/99436
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectLipoarabinomannanen_ZA
dc.subjectUrine -- Examinationen_ZA
dc.subjectMicrobiological assayen_ZA
dc.subjectMonoclonal antibodies -- Diagnostic useen_ZA
dc.titleA sensitive urinary lipoarabinomannan test for tuberculosisen_ZA
dc.typeArticleen_ZA
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