Characterization of volatiles and aroma-active compounds in honeybush (Cyclopia subternata) by GC-MS and GC-O analysis

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dc.contributor.authorLe Roux, Maritha.
dc.contributor.authorCronje, J. Christel
dc.contributor.authorBurger, B. V.
dc.contributor.authorJoubert, Elizabeth
dc.date.accessioned2012-04-12T08:33:26Z
dc.date.available2012-04-12T08:33:26Z
dc.date.issued2012-03
dc.descriptionThe original publication is available at http://pubs.acs.org/doi/full/10.1021/jf2048383en
dc.description.abstractVolatile organic compounds (VOCs) in fermented honeybush, Cyclopia subternata, were sampled by means of a high-capacity headspace sample enrichment probe (SEP) and analyzed by gas chromatography-mass spectrometry (GC-MS). Stereochemistry was determined by means of enantioselective GC-MS with derivatized β-cyclodextrin columns as chiral selectors. A total of 183 compounds, the majority of which are terpenoids (103; 56%), were identified by comparing their mass spectra and retention indices with those of reference compounds or tentatively identified by comparison with spectral library or literature data. Of these compounds, 37 were determined by gas chromatography-olfactometry (GC-O), using detection frequency (DF) and aroma extract dilution analysis (AEDA), to be odor-active (FD ≥ 2). (E)-β-Damascenone, (R/S)-linalool, (E)-β-damascone, geraniol, (E)-β-ionone, and (7E)-megastigma-5,7,9-trien-4-one were identified with the highest FD factors (≥512). The odors of certain compounds, that is, (6E,8Z)-megastigma-4,6,8-trien-3-one, (6E,8E)-megastigma-4,6,8-trien-3-one, (7E)-megastigma-5,7,9-trien-4-one, 10-epi-γ-eudesmol, epi-α- muurolol, and epi-α-cadinol, were perceived by GC-O assessors as typically honeybush-like. © 2012 American Chemical Society.
dc.description.versionPost-print
dc.format.extent16 p.
dc.identifier.citationLe Roux, M., Cronje, J. C., Burger, B. V. & Joubert, E. 2012. Characterization of volatiles and aroma-active compounds in honeybush (Cyclopia subternata) by GC-MS and GC-O analysis, Journal of agricultural and food chemistry, 60(10):2657-2644, doi:10.1021/jf2048383.
dc.identifier.issn1520-5118 (online)
dc.identifier.issn0021-8561 (print)
dc.identifier.otherdoi:10.1021/jf2048383
dc.identifier.urihttp://hdl.handle.net/10019.1/20694
dc.publisherAmerican Chemical Society
dc.rights.holderAmerican Chemical Society
dc.subjectGas chromatography-mass spectrometry (GC-MS)en_ZA
dc.subjectGas chromatography-olfactometry (GC-O)en_ZA
dc.subjectHoneybush tea -- Processingen_ZA
dc.subjectOdor-active compoundsen_ZA
dc.subjectSample enrichment probeen_ZA
dc.subjectTerpenoidsen_ZA
dc.subjectVolatile organic compoundsen_ZA
dc.titleCharacterization of volatiles and aroma-active compounds in honeybush (Cyclopia subternata) by GC-MS and GC-O analysisen_ZA
dc.typeArticleen_ZA
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