The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria

Simple item page
dc.contributor.authorGey van Pittius, Nico C.
dc.contributor.authorGamieldien, Junaid
dc.contributor.authorHide, Winston
dc.contributor.authorBrown, Gordon D.
dc.contributor.authorSiezen, Roland J.
dc.contributor.authorBeyers, Albert D.
dc.date.accessioned2010-12-14T08:43:10Z
dc.date.available2010-12-14T08:43:10Z
dc.date.issued2001-09
dc.date.updated2010-11-10T13:05:48Z
dc.descriptionIncludes bibliography
dc.description.abstractBackground: The genome of Mycobacterium tuberculosis H37Rv has five copies of a cluster of genes known as the ESAT-6 loci. These clusters contain members of the CFP-10 (lhp) and ESAT-6 (esat-6) gene families (encoding secreted T-cell antigens that lack detectable secretion signals) as well as genes encoding secreted, cell-wall-associated subtilisin-like serine proteases, putative ABC transporters, ATP-binding proteins and other membrane-associated proteins. These membrane-associated and energy-providing proteins may function to secrete members of the ESAT-6 and CFP-10 protein families, and the proteases may be involved in processing the secreted peptide. Results: Finished and unfinished genome sequencing data of 98 publicly available microbial genomes has been analyzed for the presence of orthologs of the ESAT-6 loci. The multiple duplicates of the ESAT-6 gene cluster found in the genome of M. tuberculosis H37Rv are also conserved in the genomes of other mycobacteria, for example M. tuberculosis CDC1551, M. tuberculosis 210, M. bovis, M. leprae, M. avium, and the avirulent strain M. smegmatis. Phylogenetic analyses of the resulting sequences have established the duplication order of the gene clusters and demonstrated that the gene cluster known as region 4 (Rv3444c-3450c) is ancestral. Region 4 is also the only region for which an ortholog could be found in the genomes of Corynebacterium diphtheriae and Streptomyces coelicolor. Conclusions: Comparative genomic analysis revealed that the presence of the ESAT-6 gene cluster is a feature of some high-G+C Gram-positive bacteria. Multiple duplications of this cluster have occurred and are maintained only within the genomes of members of the genus Mycobacterium.en_ZA
dc.description.versionPeer Reviewed
dc.format.extent[17 p.] : ill.
dc.identifier.citationGey van Pittius, NC, Gamieldien, J, Hide, W, Brown, GD, Siezen, RJ & Beyers, AD 2001, 'The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria', Genome Biology, 2(10):research0044-research0044.18.en_ZA
dc.identifier.issn1465-6906 (printed)
dc.identifier.issn1465-6914 (online)
dc.identifier.otherhttp://dx.doi.org/10.1186/gb-2001-2-10-research0044
dc.identifier.urihttp://hdl.handle.net/10019.1/5087
dc.language.isoen_ZAen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderGey van Pittius et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectMycobacterium tuberculosis H37Rven_ZA
dc.subjectESAT-6 locien_ZA
dc.subjectMicrobial genomesen_ZA
dc.subject.lcshGenomicsen_ZA
dc.subject.lcshCarrier proteins -- Researchen_ZA
dc.titleThe ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteriaen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
geyvanpittius_esat_2001.pdf
Size:
169.14 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Molecular Biology and Human Genetics)