DNA damage response of haematopoietic stem and progenitor cells to high-LET neutron irradiation

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dc.contributor.authorEngelbrecht, Moniqueen_ZA
dc.contributor.authorNdimba, Royaen_ZA
dc.contributor.authorDe Kock, Marynaen_ZA
dc.contributor.authorMiles, Xantheneen_ZA
dc.contributor.authorNair, Shankarien_ZA
dc.contributor.authorFisher, Randallen_ZA
dc.contributor.authorDu Plessis, Peteren_ZA
dc.contributor.authorBolcaen, Julieen_ZA
dc.contributor.authorBotha, Matthys Hendriken_ZA
dc.contributor.authorZwanepoel, Elbieen_ZA
dc.contributor.authorSioen, Simonen_ZA
dc.contributor.authorBaeyens, Ansen_ZA
dc.contributor.authorNieto-Camero, Jaimeen_ZA
dc.contributor.authorDe Kock, Evanen_ZA
dc.contributor.authorVandevoorde, Charloten_ZA
dc.date.accessioned2023-04-04T13:38:32Zen_ZA
dc.date.available2023-04-04T13:38:32Zen_ZA
dc.date.issued2021-10en_ZA
dc.descriptionCITATION: Engelbrecht, M. et al. 2021. DNA damage response of haematopoietic stem and progenitor cells to high-LET neutron irradiation. Scientific Reports, 11:20854, doi:10.1038/s41598-021-00229-2.en_ZA
dc.descriptionThe original publication is available at https://www.nature.comen_ZA
dc.description.abstractThe radiosensitivity of haematopoietic stem and progenitor cells (HSPCs) to neutron radiation remains largely underexplored, notwithstanding their potential role as target cells for radiation-induced leukemogenesis. New insights are required for radiation protection purposes, particularly for aviation, space missions, nuclear accidents and even particle therapy. In this study, HSPCs (CD34+CD38+ cells) were isolated from umbilical cord blood and irradiated with 60Co γ-rays (photons) and high energy p(66)/Be(40) neutrons. At 2 h post-irradiation, a significantly higher number of 1.28 ± 0.12 γ-H2AX foci/cell was observed after 0.5 Gy neutrons compared to 0.84 ± 0.14 foci/cell for photons, but this decreased to similar levels for both radiation qualities after 18 h. However, a significant difference in late apoptosis was observed with Annexin-V+/PI+ assay between photon and neutron irradiation at 18 h, 43.17 ± 6.10% versus 55.55 ± 4.87%, respectively. A significant increase in MN frequency was observed after both 0.5 and 1 Gy neutron irradiation compared to photons illustrating higher levels of neutron-induced cytogenetic damage, while there was no difference in the nuclear division index between both radiation qualities. The results point towards a higher induction of DNA damage after neutron irradiation in HSPCs followed by error-prone DNA repair, which contributes to genomic instability and a higher risk of leukemogenesis.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-021-00229-2en_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent13 pagesen_ZA
dc.identifier.citationEngelbrecht, M. et al. 2021. DNA damage response of haematopoietic stem and progenitor cells to high-LET neutron irradiation. Scientific Reports, 11:20854, doi:10.1038/s41598-021-00229-2.en_ZA
dc.identifier.issn2045-2322 (online)en_ZA
dc.identifier.otherdoi:10.1038/s41598-021-00229-2en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/126769en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherNatureen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectDNA damageen_ZA
dc.subjectStem cells -- Effect of radiation onen_ZA
dc.subjectNeutron radiographyen_ZA
dc.subjectLeukemia -- Etiologyen_ZA
dc.subjectNeutron irradiationen_ZA
dc.titleDNA damage response of haematopoietic stem and progenitor cells to high-LET neutron irradiationen_ZA
dc.typeArticleen_ZA
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