Detection of p53 gene mutations in oral squamous cell carcinomas of a black african population sample

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dc.contributor.authorVan Rensburg E.J.
dc.contributor.authorEngelbrecht S.
dc.contributor.authorVan Heerden W.E.P.
dc.contributor.authorKotze M.J.
dc.contributor.authorRaubenheimer E.J.
dc.date.accessioned2011-05-15T16:16:33Z
dc.date.available2011-05-15T16:16:33Z
dc.date.issued1998
dc.description.abstractMutations in the p53 gene have been reported in head and neck carcinomas. We determined the p53 mutation profile in 55 oral squamous cell carcinomas (OSCCs) from a black African population sample. DNA from all the patients were investigated using PCR amplification of the p53 gene (exons 5-9), followed by heteroduplex single-stranded conformational polymorphism (HEX-SSCP) analysis on the PCR products. Direct sequencing was performed on cases where mutations were identified. The results showed mutations in 13 of 55 (23.6%) tumours. Eleven of 13 (85%) were single base pair substitutions (9 transitions and 2 transversions), and 2 were deletions. Two novel mutations were identified: a large 63-base pair deletion, and a single base pair substitution. The mutations in our study occurred outside the head and neck tumour hot spot region (codons 238-248). © 1998 Wiley-Liss, Inc.
dc.description.versionArticle
dc.identifier.citationHuman Mutation
dc.identifier.citation11
dc.identifier.citation1
dc.identifier.issn10597794
dc.identifier.urihttp://hdl.handle.net/10019.1/13829
dc.subjectadult
dc.subjectaged
dc.subjectamino acid substitution
dc.subjectarticle
dc.subjectfemale
dc.subjectgenetics
dc.subjecthuman
dc.subjectincidence
dc.subjectmale
dc.subjectmiddle aged
dc.subjectmolecular genetics
dc.subjectmouth tumor
dc.subjectmutation
dc.subjectNegro
dc.subjectnucleotide sequence
dc.subjectSouth Africa
dc.subjectsquamous cell carcinoma
dc.subjecttumor suppressor gene
dc.subjectAdult
dc.subjectAfrican Continental Ancestry Group
dc.subjectAged
dc.subjectAmino Acid Substitution
dc.subjectBase Sequence
dc.subjectCarcinoma, Squamous Cell
dc.subjectFemale
dc.subjectGenes, p53
dc.subjectHumans
dc.subjectIncidence
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMolecular Sequence Data
dc.subjectMouth Neoplasms
dc.subjectMutation
dc.subjectSouth Africa
dc.titleDetection of p53 gene mutations in oral squamous cell carcinomas of a black african population sample
dc.typeArticle
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