Defining bioassay conditions to evaluate sperm/zona interaction: Inhibition of zona binding mediated by solubilized human zona pellucida

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dc.contributor.authorFranken D.R.
dc.contributor.authorHenkel R.
dc.contributor.authorKaskar K.
dc.contributor.authorHabenicht U.-F.
dc.date.accessioned2011-05-15T16:16:51Z
dc.date.available2011-05-15T16:16:51Z
dc.date.issued1996
dc.description.abstractObjective: Our goal was to study the influence of solubilized human zonae pellucidae on zona binding potential and acrosome reaction. Materials and Methods: Zona pellucida (ZP) solutions were prepared by dissolving zona in acidic buffer, NaH2PO4 (pH 2.5), to obtain 0.1 and 0.5 zona pellucida/μl. Zona binding capacity was evaluated by the addition of oocytes (10-fold) to sperm/zona pellucida solution droplets. The number of sperm bound to each oocyte was recorded. Zona pellucida mediated acrosome activity was evaluated after 60 min of coincubation of sperm and 0.5 ZP/μl. Results: The mean (±SE) number of sperm bound for control, 0.1 ZP/μl, and 0.5 ZP/μl was 181.2 ± 12, 79.6 ± 5, and 38.8 ± 3, respectively. Zona pellucida-exposed sperm populations showed significant more acrosome-reacted sperm compared to control sperm, namely, 78 versus 32%, respectively (P = 0.001). Conclusions: The observed zona binding inhibition might be ascribed to zona receptor blocking on the sperm surface or the inability of acrosome-reacted sperm to bind to the zona pellucida.
dc.description.versionArticle
dc.identifier.citationJournal of Assisted Reproduction and Genetics
dc.identifier.citation13
dc.identifier.citation4
dc.identifier.issn10580468
dc.identifier.other10.1007/BF02070147
dc.identifier.urihttp://hdl.handle.net/10019.1/13966
dc.subjectacrosome reaction
dc.subjectarticle
dc.subjectbinding affinity
dc.subjectbioassay
dc.subjectcell interaction
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectfertilization in vitro
dc.subjectgamete
dc.subjecthuman
dc.subjecthuman cell
dc.subjectinfertility
dc.subjectinfertility therapy
dc.subjectintracytoplasmic sperm injection
dc.subjectmale
dc.subjectmicroinjection
dc.subjectoocyte
dc.subjectpriority journal
dc.subjectsolubilization
dc.subjectsperm
dc.subjectzona pellucida
dc.subjectAcrosome
dc.subjectBiological Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectSolubility
dc.subjectSperm-Ovum Interactions
dc.subjectZona Pellucida
dc.titleDefining bioassay conditions to evaluate sperm/zona interaction: Inhibition of zona binding mediated by solubilized human zona pellucida
dc.typeArticle
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