The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes

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dc.contributor.advisorSwart, Amanda C.en_ZA
dc.contributor.advisorSwart, P.en_ZA
dc.contributor.authorPerold, Heleneen_ZA
dc.contributor.otherUniversity of Stellenbosch. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.date.accessioned2009-03-04T05:58:50Zen_ZA
dc.date.accessioned2010-06-01T08:47:30Z
dc.date.available2009-03-04T05:58:50Zen_ZA
dc.date.available2010-06-01T08:47:30Z
dc.date.issued2009-03
dc.descriptionThesis (MSc (Biochemistry))--University of Stellenbosch, 2009.
dc.description.abstractThis study: 1. Describes the preparation of unfermented and fermented rooibos methanol and aqueous extracts. 2. Investigates the influence of unfermented and fermented rooibos methanol and aqueous extracts on the binding of natural steroid substrates to ovine adrenal microsomal cytochrome P450 enzymes, demonstrating that the binding of natural steroids is inhibited in the presence of rooibos extracts. 3. Describes an assay demonstrating the inhibitory effect of rooibos extracts on the catalytic activity of cytochrome 17α-hydroxylase (CYP17) and cytochrome 21-hydroxylase (CYP21) in ovine adrenal microsomes. 4. Investigates the influence of unfermented and fermented rooibos methanol extracts on the catalytic activity of individual cytochrome P450 enzymes – CYP17 and baboon CYP21, that are expressed in COS1 cells. 5. Demonstrates that fractions of the unfermented rooibos methanol extract inhibits the binding of natural steroid substrate to microsomal cytochrome P450 enzymes as well as the catalytic activity of baboon CYP21 expressed in COS1 cells. 6. Investigates the inhibitory influence of individual rooibos flavonoids on the catalytic activity of baboon CYP21 expressed in COS1 cells.en
dc.identifier.urihttp://hdl.handle.net/10019.1/2381
dc.language.isoen
dc.publisherStellenbosch : University of Stellenbosch
dc.rights.holderUniversity of Stellenbosch
dc.subjectAspalathus linearisen_ZA
dc.subjectCytochrome P-450en_ZA
dc.subjectAdrenal steroidogenesisen_ZA
dc.subjectFlavonoidsen_ZA
dc.subjectEnzymesen_ZA
dc.subjectMetalloenzymesen_ZA
dc.subjectDissertations -- Biochemistryen_ZA
dc.subjectTheses -- Biochemistryen_ZA
dc.titleThe influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymesen_ZA
dc.typeThesisen_ZA
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