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Rapid diagnosis of cryptococcal meningitis by use of lateral flow assay on cerebrospinal fluid samples : influence of the high-dose “hook” effect

dc.contributor.authorLourens, Adreen_ZA
dc.contributor.authorJarvis, Joseph N.en_ZA
dc.contributor.authorMeintjes, Graemeen_ZA
dc.contributor.authorSamuel, Catherine M.en_ZA
dc.date.accessioned2016-06-27T09:19:48Z
dc.date.available2016-06-27T09:19:48Z
dc.date.issued2014
dc.identifier.citationLourens, A., et al. 2014. Rapid diagnosis of cryptococcal meningitis by use of lateral flow assay on cerebrospinal fluid samples: Influence of the high-dose “hook” effect. Journal of Clinical Microbiology, 52 (12): 4172-4175, doi: 10.1128/JCM.01683-14
dc.identifier.issn1098-660X (online)
dc.identifier.issn0095-1137 (print)
dc.identifier.otherdoi: 10.1128/JCM.01683-14
dc.identifier.urihttp://hdl.handle.net/10019.1/99053
dc.descriptionCITATION: Lourens, A., et al. 2014. Rapid diagnosis of cryptococcal meningitis by use of lateral flow assay on cerebrospinal fluid samples: Influence of the high-dose “hook” effect. Journal of Clinical Microbiology, 52 (12): 4172-4175, doi: 10.1128/JCM.01683-14.
dc.descriptionThe original publication is available at http://jcm.asm.org
dc.description.abstractCryptococcal meningitis is the most frequent cause of meningitis and a major cause of mortality in HIV-infected adults in Africa. This study evaluated the performance of the lateral flow assay (LFA) on cerebrospinal fluid (CSF) samples for the diagnosis of cryptococcal meningitis against that of existing diagnostic tests. LFA performed on 465 undiluted CSF samples had a sensitivity of 91%. When the LFA was paired with Gram staining, a sensitivity of 100% was achieved after implementation of a dilution step for samples with negative LFA results and the presence of yeasts on microscopy. Microscopy is essential for preventing the reporting of false-negative results due to the high-dose “hook” effect.en_ZA
dc.description.urihttp://jcm.asm.org/content/52/12/4172.abstract
dc.format.extent4 pages
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Society for Microbiology
dc.subjectMeningitis -- Diagnosis -- South Africaen_ZA
dc.subjectHIV infections -- Complication -- South Africaen_ZA
dc.subjectLateral flow testsen_ZA
dc.titleRapid diagnosis of cryptococcal meningitis by use of lateral flow assay on cerebrospinal fluid samples : influence of the high-dose “hook” effecten_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's version
dc.rights.holderAmerican Society for Microbiology


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