The optimization of the extraction and purification of horseradish peroxidase from horseradish roots

dc.contributor.advisorSwart, Pieteren_ZA
dc.contributor.advisorGraz, Michaelen_ZA
dc.contributor.authorBarnard, Almeroen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.descriptionThesis (MSc)--Stellenbosch University, 2012.en_ZA
dc.description.abstractENGLISH ABSTRACT: This study describes: a) the optimization of the current industrial-scale extraction and purification of Horseradish peroxidase from horseradish at BBI Enzymes, focussing on: a. Raw material quality, b. Extraction, c. Ultra-filtration, d. Salt fractionation, e. Diafiltration, f. Ion Exchange Chromatography, b) developing an new in-process microtitre plate calorimetric assay, c) characterization of main groups of HRP relevant to BBI Enzymes by SDS-PAGE- and HPLC analysis.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die optimisering van die huidige industriële-skaal ekstraksie en suiwering van peperwortelperoksidase vanuit peperwortel by BBI Enzymes, deur te focus op: a. Rou material kwaliteit, b. Ekstraksie, c. Ultra-filtrasie, d. Sout fraksionering, e. Diafiltrasie, f. Ioon-uitruilchromatografie b) Ontwikkeling van ‘n nuwe in-proses mikro-titer gebaseerde kalorimetriese toetsmetode c) die karakterisering van die hoof groepe peperwortel-peroksidase belangrik vir BBI Enzymes.af_ZA
dc.format.extent132 p. : ill.
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.subjectHorseradish peroxidaseen_ZA
dc.subjectEnzymes -- Purificationen_ZA
dc.subjectDissertations -- Biochemistryen_ZA
dc.subjectTheses -- Biochemistryen_ZA
dc.titleThe optimization of the extraction and purification of horseradish peroxidase from horseradish rootsen_ZA
dc.rights.holderStellenbosch University

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