Novel method for rapid measurement of growth of mycobacteria in detergent-free media
Date
1998
Authors
Meyers P.R.
Bourn W.R.
Steyn L.M.
Van Helden P.D.
Beyers A.D.
Brown G.D.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for monitoring mycobacterial growth but is particularly useful for heavily clumped cultures grown in defined minimal medium.
Description
Keywords
article, bacterial growth, bacterial membrane, bacterium identification, cell aggregation, centrifugation, mycobacterium bovis, mycobacterium smegmatis, mycobacterium tuberculosis, priority journal, quantitative diagnosis, reliability, Bacterial Proteins, Bacteriological Techniques, Culture Media, Detergents, Mycobacterium, Mycobacterium bovis, Mycobacterium tuberculosis, Sodium Hydroxide, Time Factors, Actinobacteria (class), Bacteria (microorganisms), Corynebacterineae, Mycobacterium bovis, Mycobacterium smegmatis, Mycobacterium tuberculosis, uncultured actinomycete
Citation
Journal of Clinical Microbiology
36
9
36
9