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Acrosomal morphology as a novel criterion for male fertility diagnosis: Relation with acrosin activity, morphology (strict criteria), and fertilization in vitro

dc.contributor.authorMenkveld R.
dc.contributor.authorVermeiden J.P.W.
dc.contributor.authorRhemrev J.P.T.
dc.contributor.authorKruger T.F.
dc.contributor.authorFranken D.R.
dc.date.accessioned2011-05-15T16:16:26Z
dc.date.available2011-05-15T16:16:26Z
dc.date.issued1996
dc.identifier.citationFertility and Sterility
dc.identifier.citation65
dc.identifier.citation3
dc.identifier.issn00150282
dc.identifier.urihttp://hdl.handle.net/10019.1/13784
dc.description.abstractObjective: To determine the relationships between sperm acrosin activity, sperm morphology evaluated according to strict criteria, visually observed acrosomal morphology, and IVF rates. Design: Prospective analytic study. Acrosin activity was determined on all semen samples together with a standard semen analysis. Emphasis was placed on sperm morphology and especially a novel criterion viz acrosome morphology (acrosome index) as recorded with bright field microscopy. Setting: University-based tertiary care center. Patients: Thirty-three couples undergoing IVF or GIFT with two or more metaphase II ova inseminated in vitro. Main Outcome Measure: In vitro fertilization rates of inseminated ova. Results: Strong correlations were found between acrosome index, normal sperm morphology, and IVF rates. An acrosome index cutoff value could be established at >10% normal acrosomes for IVF rates of ≥50% (sensitivity and specificity = 100%) and an acrosin activity cutoff value at >18 μIU/106 sperm. A multiple linear regression analysis showed that the acrosome index and acrosin activity added a significant contribution to the explanation of the variation in the fertilization rates. Conclusions: A strong positive correlation was found between acrosome index and IVF rates. Although the numbers of the study are small, the results indicate that the acrosome index possibly may be regarded as an additional tool in the prediction of IVF outcome and especially may be of value in the group of men with severe teratozoospermia, i.e., ≤4% morphologically normal spermatozoa.
dc.subjectacrosin
dc.subjectacrosome
dc.subjectarticle
dc.subjectcell structure
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectcorrelation function
dc.subjectfertilization in vitro
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectmale fertility
dc.subjectpriority journal
dc.subjectreproducibility
dc.subjectspermatozoon
dc.subjectAcrosin
dc.subjectAcrosome
dc.subjectFertilization
dc.subjectFertilization in Vitro
dc.subjectForecasting
dc.subjectHumans
dc.subjectInfertility, Male
dc.subjectMale
dc.subjectProspective Studies
dc.subjectRegression Analysis
dc.subjectROC Curve
dc.subjectSemen
dc.titleAcrosomal morphology as a novel criterion for male fertility diagnosis: Relation with acrosin activity, morphology (strict criteria), and fertilization in vitro
dc.typeArticle
dc.description.versionArticle


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