Validation of a new disposable counting chamber
Date
2001
Authors
Coetzee K.
Menkveld R.
Journal Title
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Volume Title
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Abstract
In a routine prospective study, 3 different counting chambers were compared for manual and computer-assisted evaluations. Swim-up samples were used so as to remove all debris and other cells that may contaminate the evaluation process when using a semen analyzer. The Makler concentration determinations (n = 20) were, on average, approximately 20 × 106 cells/mL higher compared to the corresponding 20 hemocytometer counts. The mean differences between the Leja chambers and the hemocytometer counts (n = 320) were only around 1 × 106 cells/mL, with coefficients of variation around 20%. The Leja chambers for both manual and the computer-assisted sperm concentration determinations provided consistent and accurate data on sperm concentration.
Description
Keywords
article, computer assisted diagnosis, controlled study, diagnostic accuracy, diagnostic value, human, human cell, intermethod comparison, major clinical study, male, male infertility, priority journal, semen analysis, spermatozoon count, spermatozoon density, spermatozoon motility, validation process, Humans, Male, Prospective Studies, Sperm Count
Citation
Archives of Andrology
47
2
47
2