dc.contributor.advisor | Strijdom, Hans | en_ZA |
dc.contributor.advisor | Everson, Frans | en_ZA |
dc.contributor.advisor | Kamau, Festus | en_ZA |
dc.contributor.author | Kgokane, Boipelo Mirriam Lepetjia | en_ZA |
dc.contributor.other | Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Medical Physiology. | en_ZA |
dc.date.accessioned | 2022-03-02T14:29:08Z | |
dc.date.accessioned | 2022-04-29T09:25:43Z | |
dc.date.available | 2022-03-02T14:29:08Z | |
dc.date.available | 2022-04-29T09:25:43Z | |
dc.date.issued | 2022-04 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/124674 | |
dc.description | Thesis (MSc)--Stellenbosch University, 2022. | en_ZA |
dc.description.abstract | Background and Aim
Cardiovascular disease in people living with HIV has become of great concern. HI-viral factors, ART-toxicity
and HIV/ART-associated cardiometabolic adverse effects have been implicated in the development of
cardiovascular disease. Retinal microvascular geometric features may be potential useful markers of these
effects. We aimed to investigate whether altered retinal microvascular geometric features are markers of HIV,
ART and/or HIV/ART-associated cardiometabolic effects in a study population from the Western Cape
Province.
Methods
The study followed a cross-sectional (HIV-free: n = 88 and HIV+ART: n = 122) and longitudinal (baseline vs.
18-month follow-up for HIV+ART only: n = 82) study design. Volunteering participants were recruited from
health care clinics. Demographic, lifestyle, socioeconomic and anthropometric data were collected. Fasting
blood and urine samples were collected and transported to the National Health Laboratory Services for
biochemical analyses. Retinal images were obtained (Canon CR-2 camera) and vessel features quantified
(MONA REVA 2.1.1 software). Linear stepwise regression (cross-sectional) and linear mixed model
(longitudinal) analyses were applied to elucidate independent associations and statistical significance of p <
0.05.
Results
Population characteristics: The study population was relatively young (HIV-free:44.06±11.09 and
HIV+ART:40.35±8.94years) and mostly women (HIV-free:80.7% and HIV+ART:63.1%). The baseline
median/mean viral load (VL), CD4 cell count and ART-duration were 50 (10 to 675032) copies mRNA/mL,
539.92±237.16 cells/mm3 and 166 (1 to 707) weeks respectively. Cardiometabolic results: Body mass index
(BMI) (24.50±6.65 vs. 28.25±7.68kg/m2, p < 0.001) was significantly lower in HIV+ART vs. HIV-free. ∆BMI
in HIV+ART was significantly correlated with average arterial tree diameter (r = 0.323, p < 0.05), total length
of skeletonised tree (r = 0.355, p < 0.01) and arteriolar branching angle (r = 0.234, p < 0.05). High density
lipoprotein-cholesterol (1.59±0.74 vs. 1.39±0.45mmol/L, p = 0.019) and gamma-glutamyl transferase (GGT)
(43.5 (14 to 494) vs. 27.0 (11 to 814)U/L, p < 0.001) were significantly higher in HIV+ART vs. HIV-free, but
decreased in HIV+ART (Baseline vs. Follow-up HDL:1.62±0.77 vs. 1.44±0.64mmol/L, p = 0.017 and GGT:45
(14 to 494) vs. 41.50 (14 to 219)U/L, p = 0.004). HDL was significantly correlated with central retinal venular
equivalent (CRVE) (r =-0.195, p < 0.01) and GGT with venular branching optimality (r = 0.180, p < 0.05).
HIV and ART results: Cross-sectionally, HIV+ART status independently associated with CRVE (-0.146 (-
0.280 to -0.012), p = 0.033) and arteriolar and venular mother branch (D0), first daughter branch (D1) and
second daughter branch (D2) (p < 0.05, respectively). VL (-0.198 (-0.025 to -0.001), p = 0.037) and ART-
duration (0.188 (0.001 to 0.024), p = 0.047) were independently associated with arteriolar-venular ratio (AVR).
Longitudinally, VL independently associated with CRVE (0.096 (0.017 to 0.175), p = 0.018) and AVR (-0.003
(-0.0006 to 0.000003), p = 0.046). CD4 cell count was independently associated with number of branchpoints 0.042 (-0.002 to 0.086), p=0.006) and endpoints (3.0 (0.750 to 5.250), p=0.010). HIV duration independently
associated with lacunarity (-0.0080 (-0.0150 to -0.0010), p=0.036) and fractal analyses (0.011 (0.0001 to
0.021), p=0.045). 2nd-line ART was independently associated with CRVE (8.58 (0.35 to 16.81), p=0.041) and
ART-duration with fractal analysis (-0.022 (-0.037 to -0.008), p=0.003).
Discussion and conclusion
HIV+ART appeared to have a more favourable cardiovascular risk profile vs. HIV-free. Various markers of
HIV/ART and HIV-ART-associated cardiometabolic risk factors were associated with retinal vessel features
and associations appeared mostly favourable/cardioprotective. These results indicate that retinal vessel
geometric features may be potential markers of the effects of HIV/ART and/or associated cardiometabolic risk
factors in the current study population. | af_ZA |
dc.description.abstract | Agtergrond en Doelstelling
Kardiovaskulêre siekte in MIV-positiewe individue wek groot kommer. MI-virale faktore, antiretrovirale
terapie (ART)-toksisiteit en MIV/ART-geassosieerde kardiometaboliese newe-effekte word geïmpliseer.
Retinale mikrovaskulêre geometriese patrone mag potensieël nuttige merkers van hierdie effekte wees. Die
doel van hierdie studie was om te ondersoek of veranderinge in retinale mikrovaskulêre geometriese patrone
as merkers van MIV, ART en/of MIV/ART-geassosieerde kardiometaboliese effekte in ‘n studie populasie
van die Wes-Kaap beskou kan word.
Metodes
Die studie het ‘n deursnee (MIV-vry: n = 88 en MIV+ART: n = 122) en longitudinale (basislyn vs. 18-maande
opvolg vir slegs MIV+ART: n = 82) studie-ontwerp gevolg. Vrywillige deelnemers was vanaf gesondheidsorg
klinieke gewerf. Demografiese, lewenstyl, sosio-ekonomiese en antropometriese inligting is ingewin.
Vastende bloed- en urinemonsters was versamel en na die NHLS vervoer vir biochemiese ontledings. Retinale
beelde was vasgelê (Canon CR2-kamera) en bloedvat patrone was gekwantifiseer (MONA REVA 2.1.1
sagteware). Liniêre, stapsgewyse regressie (deursnee studie) en liniêre gemengde model (longitudinale studie)
analises was aangewend om onafhanklike assosiasies te ondersoek (statistiese beduidenheid: p<0.05).
Resultate
Populasie eienskappe: Die studie populasie was relatief jonk (MIV-vry:44.06±11.09 en
MIV+ART:40.35±8.94 jaar) en het meestal uit vroue bestaan (MIV-vry:80.7% and MIV+ART:63.1%). Die
basislyn mediaan/gemiddelde virale lading (VL), CD4 seltelling en ART-duur was onderskeidelik 50 (10 tot
675032) kopieë bRNA/mL, 539.92±237.16 selle/mm3 and 166 (1 to 707) weke. Kardiometaboliese resultate:
Liggaam-massa-indeks (BMI) (24.50±6.65 vs. 28.25±7.68kg/m2, p<0.001) was betekenisvol laer in
MIV+ART vs. MIV-vry. ∆BMI in MIV+ART was betekenisvol gekorreleer met die gemiddelde arteriële boom
deursnit (r=0.323, p<0.05), totale lengte van die skelet boom (r=-0.355, p<0.01) en arteriolêre vertakkingshoek
(r=0.234, p<0.05). HDL-cholesterol (1.59±0.74 vs. 1.39±0.45mmol/L, p=0.019) en GGT (43.5 (14 to 494) vs.
27.0 (11 to 814) U/L, p<0.001) was betekenisvol hoër in MIV+ART vs. MIV-vry, maar laer in MIV+ART
(Basislyn vs. Opvolg HDL:1.62±0.77 vs. 1.44±0.64mmol/L, p=0.017 en GGT:45 (14 to 494) vs. 41.50 (14 tot
219)U/L, p=0.004). HDL het betekenisvol gekorreleer met CRVE (r=-0.195, p<0.01), en GGT met venulêre
vertakking (r=0.180, p<0.05). MIV en ART resultate: Deursnee studie: MIV+ART status was onafhanklik
geassosieer met CRVE (-0.146 (-0.280 tot -0.012), p=0.033), en arteriolêre en venulêre D0, D1 en D2 (p<0.05
onderskeidelik). VL (-0.198 (-0.025 tot -0.001), p = 0.037) and ART-duur (0.188 (0.001 tot 0.024), p=0.047)
het onafhanklik met AVR geassosieer. Longitudinale studie: VL was onafhanklik geassosieer met CRVE
(0.096 (0.017 tot 0.175), p=0.018) en AVR (-0.003 (-0.0006 tot 0.000003), p=0.046). CD4 telling was
onafhanklik geassosieer met die aantal takpunte (0.042 (-0.002 tot 0.086), p=0.006) en eindpunte (3.0 (0.750 to 5.250), p=0.010). MIV-duur was onafhanklik geassosieer met lakunariteit (-0.0080 (-0.0150 tot -0.0010),
p=0.036) en fraktale analises (0.011 (0.0001 tot 0.021), p=0.045). Tweede-lyn ART was onafhanklik geassosieer met CRVE (8.58 (0.35 tot 16.81), p=0.041) en ART-duur met fraktale analise (-0.022 (-0.037 tot
-0.008), p=0.003).
Bespreking en gevolgtrekking
MIV+ART individue blyk oor die algemeen ‘n meer gunstige kardiovaskulêre profiel te toon as mense sonder
MIV. Verskeie merkers van MIV/ART en MIV-ART-geassosieerde kardiometaboliese risiko faktore was met
retinale bloedvat eienskappe geassosieer, en die assosiasies was meestal gunstig / kardiobeskermend. Hierdie
resultate dui daarop dat retinale bloedvat geometriese eienskappe as potensiële merkers van die effekte van
MIV/ART en/of geassosieerde kardiometaboliese risiko faktore in die huidige studie populasie beskou kan
word. | af_ZA |
dc.format.extent | 133 pages | en_ZA |
dc.language.iso | en_ZA | en_ZA |
dc.publisher | Stellenbosch : Stellenbosch University | en_ZA |
dc.subject | Metabolic heart disease -- Risk factors | en_ZA |
dc.subject | UCTD | en_ZA |
dc.subject | People living with HIV/AIDS | en_ZA |
dc.subject | Microvascular disorders | en_ZA |
dc.subject | Geometric morphology | en_ZA |
dc.subject | Retinal microvascular | en_ZA |
dc.title | Cardio-metabolic risk profile of people living with HIV: Is retinal microvascular geometric morphology a marker of effect? | en_ZA |
dc.type | Thesis | en_ZA |
dc.description.version | Masters | en_ZA |
dc.rights.holder | Stellenbosch University | en_ZA |