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Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch

dc.contributor.authorCripwell, Rosemary A.en_ZA
dc.contributor.authorRose, Shaunita H.en_ZA
dc.contributor.authorFavaro, Lorenzoen_ZA
dc.contributor.authorVan Zyl, Willem H.en_ZA
dc.date.accessioned2019-08-26T07:37:27Z
dc.date.available2019-08-26T07:37:27Z
dc.date.issued2019-08-20
dc.identifier.citationCripwell, R. A., et al. 2019. Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch. Biotechnology for Biofuels, 12:201, doi:10.1186/s13068-019-1541-5en_ZA
dc.identifier.issn1754-6834 (online)
dc.identifier.otherdoi:10.1186/s13068-019-1541-5
dc.identifier.urihttp://hdl.handle.net/10019.1/106373
dc.descriptionCITATION: Cripwell, R. A., et al. 2019. Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch. Biotechnology for Biofuels, 12:201, doi:10.1186/s13068-019-1541-5.en_ZA
dc.description.abstractBackground: Consolidated bioprocessing (CBP) combines enzyme production, saccharification and fermentation into a one-step process. This strategy represents a promising alternative for economic ethanol production from starchy biomass with the use of amylolytic industrial yeast strains. Results: Recombinant Saccharomyces cerevisiae Y294 laboratory strains simultaneously expressing an α-amylase and glucoamylase gene were screened to identify the best enzyme combination for raw starch hydrolysis. The codon optimised Talaromyces emersonii glucoamylase encoding gene (temG_Opt) and the native T. emersonii α-amylase encoding gene (temA) were selected for expression in two industrial S. cerevisiae yeast strains, namely Ethanol Red™ (hereafter referred to as the ER) and M2n. Two δ-integration gene cassettes were constructed to allow for the simultaneous multiple integrations of the temG_Opt and temA genes into the yeasts’ genomes. During the fermentation of 200 g l−1 raw corn starch, the amylolytic industrial strains were able to ferment raw corn starch to ethanol in a single step with high ethanol yields. After 192 h at 30 °C, the S. cerevisiae ER T12 and M2n T1 strains (containing integrated temA and temG_Opt gene cassettes) produced 89.35 and 98.13 g l−1 ethanol, respectively, corresponding to estimated carbon conversions of 87 and 94%, respectively. The addition of a commercial granular starch enzyme cocktail in combination with the amylolytic yeast allowed for a 90% reduction in exogenous enzyme dosage, compared to the conventional simultaneous saccharification and fermentation (SSF) control experiment with the parental industrial host strains. Conclusions: A novel amylolytic enzyme combination has been produced by two industrial S. cerevisiae strains. These recombinant strains represent potential drop-in CBP yeast substitutes for the existing conventional and raw starch fermentation processes.en_ZA
dc.description.urihttps://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-019-1541-5
dc.format.extent16 pages : illustrationsen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherBMC (part of Springer Nature)en_ZA
dc.subjectBioethanol productionen_ZA
dc.subjectEthanol production from starchy biomassen_ZA
dc.subjectSaccharomyces cerevisiae strainsen_ZA
dc.subjectConversion of starch to ethanolen_ZA
dc.subjectConsolidated bioprocessing (CBP)en_ZA
dc.titleConstruction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starchen_ZA
dc.typeArticleen_ZA
dc.date.updated2019-08-25T03:28:33Z
dc.description.versionPublisher's versionen_ZA
dc.rights.holderThe Author(s)en_ZA
dc.rights.holderAuthors retain copyrighten_ZA


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