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Rv1460, a SufR homologue, is a repressor of the suf operon in mycobacterium tuberculosis

dc.contributor.authorWillemse, Danickeen_ZA
dc.contributor.authorWeber, Brandonen_ZA
dc.contributor.authorMasino, Lauraen_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.contributor.authorAdinolfi, Salvatoreen_ZA
dc.contributor.authorPastore, Annalisaen_ZA
dc.contributor.authorWilliams, Monique J.en_ZA
dc.date.accessioned2018-07-30T10:38:01Z
dc.date.available2018-07-30T10:38:01Z
dc.date.issued2018
dc.identifier.citationWillemse, D., et al. 2018. Rv1460, a SufR homologue, is a repressor of the suf operon in mycobacterium tuberculosis. PLoS ONE, 3(7):e0200145, doi:10.1371/journal.pone.0200145
dc.identifier.urihttp://hdl.handle.net/10019.1/104207
dc.descriptionCITATION: Willemse, D., et al. 2018. Rv1460, a SufR homologue, is a repressor of the suf operon in mycobacterium tuberculosis. PLoS ONE, 3(7):e0200145, doi:10.1371/journal.pone.0200145.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractIron–sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv1460, shares homology with the cyanobacterial SufR, which functions as a transcriptional repressor of the sufBCDS operon. Rv1460’s function in M. tuberculosis has however not been determined. In this study, we demonstrate that M. tuberculosis mutants lacking a functional Rv1460 protein are impaired for growth under standard culture conditions. Elevated expression of Rv1460 and Rv1461 was observed in the mutant, implicating Rv1460 in the regulation of the suf operon. Binding of an Fe-S cluster to purified recombinant Rv1460 was confirmed by UV-visible spectroscopy and circular dichroism. Furthermore, three conserved cysteine residues, C203, C216 and C244, proposed to provide ligands for the coordination of an Fe-S cluster, were shown to be required for the function of Rv1460 in M. tuberculosis. Rv1460 therefore seems to be functionally analogous to cyanobacterial SufR.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200145
dc.format.extent27 pages
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Science
dc.subjectMycobacterium tuberculosisen_ZA
dc.titleRv1460, a SufR homologue, is a repressor of the suf operon in mycobacterium tuberculosisen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's version
dc.rights.holderAuthors retain copyright


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