The effect of salsola tuberculatiformis bostchantzev on the androgen and glucocorticoid receptors in prostate cancer cell lines

Kelbe, Lisa Suzanne (2017-12)

Thesis (MSc)--Stellenbosch University, 2017.

Thesis

ENGLISH ABSTRACT: This study describes: 1. The preparation of a biologically active methanol extract from S.tuberculatiformis. 2. The demonstration of biological activity for both the crude S.tuberculatiformis extract and Compound A. 3. The effects of the crude S.tuberculatiformis extract and Compound A on nuclear translocation of the androgen and estrogens receptors in three different prostate cancer cell-lines. 4. Cell viability assays demonstrating the potency (IC50) for Compound A and the crude S.tuberculatiformis extract between the three prostate cancer cell-lines. 5. Caspase assays to demonstrate apoptosis for the crude S.tuberculatiformis extract and Compound A in prostate cancer celllines.

AFRIKAANSE OPSOMMING: Hierdie studie beskryf: 1. Die bereiding van 'n biologies-aktiewe kru metanolekstrak uit S.tuberculatiformis. 2. Die bepaling van die biologiese aktiwiteit vir beide die kru metanolekstrak uit S.tuberculatiformis en verbinding A. 3. Die effek van die kru S.tuberculatiformis ekstrak en verbindingA op die translokasie van die glukokortikoïed- en androgeenreseptore in drie verskillende prostaatkanker sel-lyne. 4. Sellewensvatbaarheidstoetse (IC50) met drie prostaatkankersellyne om die toksisiteit van die kru metanolekstrak uit S.tuberculatiformis en verbinding A te bepaal. 5. Kaspasetoetse in prostaatkanker sel-lyne om die apoptotiese effek van die kru metanolekstrak uit S.tuberculatiformis en verbinding A te demonstreer.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/102666
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