The ontogeny of somatic embryos from in vitro cultured grapevine anthers
Date
1990
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
South African Society for Enology and Viticulture
Abstract
Translucent light green to yellow anthers excised from Vitis rupestris cv. Rupestris du Lot flower buds, produced from cuttings grown in a climate-controlled room, produced somatic embryos when cultured on modified Nitsch & Nitsch (1969) medium supplemented with l μM 6-benzylaminopurine (BAP) and 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Anatomical features of developing embryogenic explants were studied at various intervals, before and after the start of in vitro culture, using light microscopy. Results indicated that callus was entirely of somatic origin. Embryogenic cells were apparently present before in vitro culture commenced and multiplied sufficiently within the first 15 days' culture to form visible embryogenic growth centres. Embryogenic callus formed from the lateral and abaxial walls of the anther, all connective tissues and the filament. Somatic embryos were observed after 60 days of culture and possessed primordial vascular tissues and secondary embryoids. No vascular connections between somatic embryos were observed.
Description
CITATION: Newton, D. J. & Goussard, P. G.. 1990. The ontogeny of somatic embryos from in vitro cultured grapevine anthers. South African Journal of Enology & Viticulture, 11(2):70-75, doi:10.21548/11-2-2271.
The original publication is available at http://www.journals.ac.za/index.php/sajev
The original publication is available at http://www.journals.ac.za/index.php/sajev
Keywords
Somatic embryos -- Anatomical ontogeny, Somatic embryos -- Morphological ontogeny, Embryogenic explants -- Anatomical features
Citation
Newton, D. J. & Goussard, P. G.. 1990. The ontogeny of somatic embryos from in vitro cultured grapevine anthers. South African Journal of Enology & Viticulture, 11(2):70-75, doi:10.21548/11-2-2271