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Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone

dc.contributor.authorJacobs, Frans Alexanderen_ZA
dc.contributor.authorSadie-Van Gijsen, Hanelen_ZA
dc.contributor.authorVan de Vyver, Marien_ZA
dc.contributor.authorFerris, William Franken_ZA
dc.date.accessioned2017-01-26T10:01:05Z
dc.date.available2017-01-26T10:01:05Z
dc.date.issued2016-08-03
dc.identifier.citationJacobs, F. A., Sadie-Van Gijsen, H., Van de Vyver, M. & Ferris, W. F. 2016. Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. Frontiers in Endocrinology, 7:108, doi:10.3389/fendo.2016.00108.
dc.identifier.issn1664-2392 (online)
dc.identifier.otherdoi:10.3389/fendo.2016.00108
dc.identifier.urihttp://hdl.handle.net/10019.1/100543
dc.descriptionCITATION: Jacobs, F. A., Sadie-Van Gijsen, H., Van de Vyver, M. & Ferris, W. F. 2016. Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. Frontiers in Endocrinology, 7:108, doi:10.3389/fendo.2016.00108.
dc.descriptionThe original publication is available at http://journal.frontiersin.org/journal/endocrinolog
dc.description.abstractENGLISH ABSTRACT: Glucocorticoid-induced osteoporosis (GIO) is associated with an increase in bone marrow adiposity, which skews the differentiation of mesenchymal stem cell (MSC) progenitors away from osteoblastogenesis and toward adipogenesis. We have previously found that vanadate, a non-specific protein tyrosine phosphatase inhibitor, prevents GIO in rats, but it was unclear whether vanadate directly influenced adipogenesis in bone-derived MSCs. For the present study, we investigated the effect of vanadate on adipogenesis in primary rat MSCs derived from bone marrow (bmMSCs) and from the proximal end of the femur (pfMSCs). By passage 3 after isolation, both cell populations expressed the MSC cell surface markers CD90 and CD106, but not the hematopoietic marker CD45. However, although variable, expression of the fibroblast marker CD26 was higher in pfMSCs than in bmMSCs. Differentiation studies using osteogenic and adipogenic induction media (OM and AM, respectively) demonstrated that pfMSCs rapidly accumulated lipid droplets within 1 week of exposure to AM, while bmMSCs isolated from the same femur only formed lipid droplets after 3 weeks of AM treatment. Conversely, pfMSCs exposed to OM produced mineralized extracellular matrix (ECM) after 3 weeks, compared to 1 week for OM-treated bmMSCs. Vanadate (10 μM) added to AM resulted in a significant reduction in AM-induced intracellular lipid accumulation and expression of adipogenic gene markers (PPARγ2, aP2, adipsin) in both pfMSCs and bmMSCs. Pharmacological concentrations of glucocorticoids (1 μM) alone did not induce lipid accumulation in either bmMSCs or pfMSCs, but resulted in significant cell death in pfMSCs. Our findings demonstrate the existence of at least two fundamentally different MSC depots within the femur and highlights the presence of MSCs capable of rapid adipogenesis within the proximal femur, an area prone to osteoporotic fractures. In addition, our results suggest that the increased bone marrow adiposity observed in GIO may not be solely due to direct effect of glucocorticoids on bone-derived MSCs, and that an increase in femur lipid content may also arise from increased adipogenesis in MSCs residing outside of the bone marrow niche.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Geen opsomming beskikbaaraf_ZA
dc.description.urihttp://journal.frontiersin.org/article/10.3389/fendo.2016.00108/full
dc.format.extent12 pagesen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Media
dc.subjectGlucocorticoidsen_ZA
dc.subjectMesenchymal stem cellsen_ZA
dc.subjectBone marrow -- Adiposityen_ZA
dc.subjectOsteoporosisen_ZA
dc.subjectVanadatesen_ZA
dc.titleVanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within boneen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's version
dc.rights.holderAuthors retain copyright


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