(Stellenbosch : Stellenbosch University, 2003-12) Douglas, C. A. (Claire Anne); Sandra, P. J. F.; Lynen, F.; Stellenbosch University. Faculty of Science. Dept. of Chemistry & Polymer Science .
ENGLISH ABSTRACT: In this study, the analysis of ammo acids usmg High Performance Liquid
Chromatography (HPLC) with pre-column derivatisation was optimised. The
derivatisation reagents include o-phthaldialdehyde (OPA), 9-
fluorenylmethylchloroformate (FMOC) and iodoacetic acid (IDA). Detection was
performed using UV and fluorescence in series. The developed method was utilised
for the analysis and quantitation of amino acids in eighteen wines. The application of
chemometric data evaluation was initiated.