Browsing by Author "Taylor, Angela E."
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- ItemData comparing the separation and elution of vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometer (UPSFC-MS/MS) compared to liquid chromatography (LC) and data presenting approaches to UPSFC method optimization(Elsevier, 2018) Jenkinson, Carl; Taylor, Angela E.; Storbeck, Karl-Heinz; Hewison, MartinThe data presented is related to the research article "Analysis of multiple vitamin D metabolites by ultra performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS)" (Jenkinson et al., 2018) [1]. This article will include data obtained from method development, optimization and analysis of multiple vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometry (UPSFC-MS/MS). This includes chromatograms from column screening to confirm the most suitable column for analyte separation. Additionally, further chromatograms and figures compare separation and analyte signal strength during the optimization of other UPSFC parameters. Mass spectra will demonstrate the optimization of MS conditions for the UPSFC-MS/MS method. Chromatogram data from UHPLC vitamin D analysis is also presented in order to compare the separation and elution of vitamin D metabolites using UPSFC and UHPLC. This data will highlight the outputs that aid in method development and identifying the separation technique suited for vitamin D quantitation.
- ItemHuman steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes : a comprehensive review(Elsevier, 2019-07-27) Schiffer, Lina; Barnard, Lise; Baranowski, Elizabeth S.; Gilligan, Lorna C.; Taylor, Angela E.; Arlt, Wiebke; Shackleton, Cedric H. L.; Storbeck, Karl-HeinzAdvances in technology have allowed for the sensitive, specific, and simultaneous quantitative profiling of steroid precursors, bioactive steroids and inactive metabolites, facilitating comprehensive characterization of the serum and urine steroid metabolomes. The quantification of steroid panels is therefore gaining favor over quantification of single marker metabolites in the clinical and research laboratories. However, although the biochemical pathways for the biosynthesis and metabolism of steroid hormones are now well defined, a gulf still exists between this knowledge and its application to the measured steroid profiles. In this review, we present an overview of steroid hormone biosynthesis and metabolism by the liver and peripheral tissues, specifically highlighting the pathways linking and differentiating the serum and urine steroid metabolomes. A brief overview of the methodology used in steroid profiling is also provided.