Browsing by Author "Parida, Shreemanta"

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    An evaluation of commercial fluorescent bead-based luminex cytokine assays
    (Public Library of Science, 2008-07-02) Siawaya, Joel Fleury Djoba; Roberts, Teri; Babb, Chantal; Black, Gillian; Golakai, Hawa Jande; Stanley, Kim; Bapela, Nchinya Bennedict; Hoal, Eileen; Parida, Shreemanta; Van Helden, Paul; Walzl, Gerhard
    The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 μl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex cytokine fluorescent bead-based immunoassays (Bio-Rad's Cytokine 17-piex kit; LINCO Inc's 29-plex kit; and RnD System's Fluorokine-Multi Analyte Profiling (MAP) base kit A and B). The LINEO Inc kit was found to be the most sensitive assay for measuring concentrations of multiple recombinant cytokines in samples that had been spiked with serial dilutions of the standard provided by the manufacturer, followed respectively by the RnD Fluorokine-(MAP) and Bio-Rad 17-plex kits. A positive correlation was found in the levels of IFN-γ measured in antigen stimulated whole blood culture supernatants by the UNCO inc 29-plex, RnD Fluorokine-(MAP) and RnD system IFN-γ Quantikine ELISA kits across a panel of controls and stimulated samples. Researchers should take the limitation of such multiplexed assays into account when planning experiments and the most appropriate use for these tests may currently be as screening tools for the selection of promising markers for analysis by more sensitive techniques. © 2008 Djoba Siawaya et al.
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    Toll-like receptor pathways in the immune responses to mycobacteria
    (Elsevier, 2004-08) Quesniaux, Valerie; Fremond, Cecile; Jacobs, Muazzam; Parida, Shreemanta; Nicolle, Delphine; Yeremeev, Vladimir; Bihl, Franck; Erard, Francois; Botha, Tania; Drennan, Michael; Soler, Marie-Noelle; Le Bert, Marc; Schnyder, Bruno; Ryffel, Bernhard
    The control of Mycobacterium tuberculosis infection depends on recognition of the pathogen and the activation of both the innate and adaptive immune responses. Toll-like receptors (TLR) were shown to play a critical role in the recognition of several pathogens. Mycobacterial antigens recognise distinct TLR resulting in rapid activation of cells of the innate immune system. Recent evidence from in vitro and in vivo investigations, summarised in this review demonstrates TLR-dependent activation of innate immune response, while the induction of adaptive immunity to mycobacteria may be TLR independent. © 2004 Elsevier SAS. All rights reserved.