Browsing by Author "Mbangcolo, Mongezi Morrison"
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- ItemAspects of honeybush tea (Cyclopia species) propagation(Stellenbosch : Stellenbosch University, 2008-12) Mbangcolo, Mongezi Morrison; Reinten, E.; Agenbag, G. A.; Stellenbosch University. Faculty of AgriSciences. Dept. of Agronomy.Honeybush (Cyclopia spp. Fabaceae) is indigenous to the fynbos botanical biome of the Eastern and Western Cape of South Africa. The increase in the international demand for honeybush tea for health benefits, concern over exploitation of wild populations and the lack of published agronomic information necessitated this study to evaluate different aspects of honeybush propagation. The main objectives of this study were to evaluate the effect of species and cutting position on rooting of cuttings of Cyclopia species using different rooting hormones, to evaluate the effect of an organic plant fertilizer and cutting position on growth and establishment of rooted cuttings and to study the influence of different seed pre-treatments on germination of Cyclopia species. Terminal and sub-terminal cuttings of C. intermedia and C. genistoides treated with different rooting hormones were rooted under day/night temperature controlled glasshouse conditions. Intermittent mist was used as means of moisture supply to the cuttings for 45-60 seconds daily every 30 minutes. C. genistoides rooted significantly better compared to C. intermedia as measured by rooting percentage, number of roots per cutting, length of longest root and mean root length during the summer season. The cutting position had a significant effect on rooting of the cuttings in summer compared to winter and spring season. The interactive effect of species, treatment and cutting position resulted into 86% of rooting in summer from the terminal cuttings of C. genistoides, while only 4% was recorded as the highest rooting percentage in both winter and spring seasons. The highest number of roots and the greatest root length per cutting were obtained with 2 and 4 g L-1 IBA from terminal cuttings of C. genistoides and these hormone concentrations were not significantly different to each other. To evaluate the effect of an organic plant fertilizer and cutting position on plant growth and establishment, rooted cuttings of two Cyclopia species (C. intermedia and C. genistoides) from two cutting positions (terminal and sub-terminal) were transferred to pots (576 cm3) and treated with Nitrosol fertilizer at application rates of 3.33 ml.L-1, 1.67 ml.L-1 and 0 ml.L-1 (control). Cyclopia plantlets were uniformly inoculated once with a symbiotic Rhizobium bacteria to improve the formation of nodules. Nitrosol® at 3.33 ml.L-1 significantly affected fresh and dry plant weight, fresh and dry root weight, number of shoots and nodules per plant compared to either 1.67 ml.L-1 or the control. Relative to species, C. genistoides performed better in terms of fresh and dry plant weight, fresh and dry root weight, and number of shoots and nodules per plant compared to C. intermedia. The origin of the cutting position did not significantly affect the above mentioned parameters. Plant mineral analysis revealed that most of the essential elements increased with increasing Nitrosol® application rates, with C. genistoides having higher levels of mineral elements than C. intermedia. This could be an indication of the differences between the two species in terms of nutrient uptake, utilization and distribution within the plant tissues. In the germination studies, seeds obtained from different seed sources of Cyclopia species were subjected to different pre-sowing treatments. Seed treatments were sulphuric acid (95%), hot water (100°C), water with smoke paper disk, and demineralised water (control). The study revealed that all the treatments had a significant effect on germination with the exception of eight year old seeds obtained from C. subternata (seed source two). Although hot water treatment improved germination compared to smoked paper disk and the control, seeds treated with hot water degenerated rapidly. The highest overall germination (77.33%) was found with one year old seeds compared to other seed sources older than one year. Although smoked paper disks generally did not improve germination compared to the control, in one year old seeds from seed source one, this treatment greatly influenced germination, suggesting that seed age might have influenced germination of these seeds. In terms of germination rate, germination generally started after four days in most treatments.
- ItemThe influence of granular and liquid top-dressed nitrogen on nitrogen use efficiency (NUE), grain yield and quality parameters of spring wheat (Triticum aestivum L.)(Stellenbosch : Stellenbosch University, 2018-12) Mbangcolo, Mongezi Morrison; Pieterse, P. J.; Stellenbosch University. Faculty of AgriSciences. Dept. of Agronomy.ENGLISH ABSTRACT: Nitrogen use efficiency (NUE) of major cereal grains including wheat (Triticum aestivum L.) is estimated to be approximately 50% due to losses from leaching, soil denitrification, gaseous plant emissions, volatilization and surface runoff. Use of liquid nitrogen fertiliser to improve grain yields and quality parameters and N use efficiencies has demonstrated positive results; however, responses are inconsistent. Low N use efficiencies indicate the need to improve crop N recoveries and possible lower environmental pollution and the already high production costs. Studies on application of granular and liquid N topdressings to wheat are limited in South Africa. Studies were conducted from 2013-2015 to evaluate the response of NUE, yield and quality parameters of spring wheat to granular (broadcast) and liquid (sprayed) N topdressings under field conditions at two locations (Roodebloem - 34o 13’31.55”S; 19o 26’13.76”E and Langgewens - 33° 16' 33.96" S; 18° 42' 14.4" E) of the Western Cape Province, and controlled glasshouse conditions (2013, 2014 and 2016, and 2014, 2015 and 2016) at the Department of Agronomy, University of Stellenbosch, South Africa. Following applications of N as limestone ammonium nitrate (LAN 28%) at 30 kg N ha-1 at sowing, granular [(LAN (28%), granular urea (46%)] and liquid [urea ammonium nitrate (UAN 32%), liquid urea (46%) solution] N topdressings (30 and 60 kg N ha-1) were applied by means of single (tillering), and split (tillering and flowering) application on spring wheat. The field study results showed that the interaction between locality and growing season significantly affected NUE and grain yields and Roodebloem showed significantly better responses in grain yield in two (2014 and 2015) of the three study years compared to Langgewens. The effect of N rate showed that higher mean grain yield was produced through the application of N at 60 kg ha-1 (3 920 kg ha-1) compared to 30 kg ha-1 (3 577 kg ha-1) at Langgewens in 2013. The N rate x method of N application interaction showed that grain yield was significantly improved by liquid N topdressing at 30 kg ha-1 compared to granular N at 30 kg N ha-1 and liquid N at 60 kg N ha-1. Roodebloem (3 090 kg.ha-1) produced significantly higher mean grain yield compared to Langgewens (2 084 kg ha-1). The protein content and falling number were not significantly affected by N treatment. In the first glasshouse experiment, UAN applied at 60 kg N ha-1 significantly improved grain yield compared to other treatments. The responses showed that 60 kg N ha-1 promoted significantly higher yields and yield parameters compared to 30 kg N ha-1 and that liquid N topdressings were superior compared to granular applied N throughout the study. Similarly, in the second glasshouse experiment, plant responses increased with increasing N rates. The method x timing interaction showed significant differences due to timing of N application for liquid N topdressings. Plants treated with liquid N once at tillering showed superior responses compared to split applications of liquid N. NUE studies showed that different N use efficiency parameters were significantly improved by liquid N topdressings where the effects were significant both under field and glasshouse conditions. Seasonal rainfall was overall the main contributing source of variation in the studies conducted under field conditions .