Browsing by Author "Holinirina Dina Miora, Ratsimandresy"
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- ItemPoint spread function engineering for fluorescence microscopy(Stellenbosch : Stellenbosch University, 2018-03) Holinirina Dina Miora, Ratsimandresy; Bosman, Gurthwin W.; Rohwer, Erich G.; Stellenbosch University. Faculty of Science. Dept. of Physics.ENGLISH SUMMARY: A tool for biological imaging is developed within this work. It consists of engineering the point spread function (PSF) of a fluorescent molecule or a nanoparticle emitter by modulating the phase of the fluorescence emission. The engineered PSF developed in this current work is called the double helix point spread function (DH-PSF). Information about the three dimensional position of an emitter and its orientation can be extracted using this PSF with a very high precision and accuracy. Other modalities, such as the pyramidal PSF and the bisected PSF, are also simulated and compared theoretically with the efficiency of the DH-PSF.