Browsing by Author "Engelbrecht, Marzanne"

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    Gluten quantification methods and their compliance with the food safety legislation of South Africa.
    (Stellenbosch : Stellenbosch University, 2023-03) Engelbrecht, Marzanne; Krugel, Maricel; Tait, Timo; Dhanani, Karim; Stellenbosch University. Faculty of Agrisciences. Dept. of Food Science.
    ENGLISH ABSTRACT: Celiac disease is an adverse food reaction elicited by the seed-storage protein called gluten. Consequently, regulatory bodies have implemented legislative requirements for labelling gluten-free products and the testing thereof. However, during the last decade various researchers have indicated that food processing creates analytical challenges, such as reduced solubility and extractability of gluten protein from the food matrix, for reliable and accurate quantitation. These challenges, therefore, may cause a food safety risk for individuals suffering from celiac disease. The management of celiac disease is thus highly dependent on accurately quantifying gluten and labelling the product according to the food safety legislation of South Africa. This study describes: 1. The quantification of gluten in processed food samples as stipulated by the South African regulations relating to labelling and advertising of foodstuffs, R.146 of 1 March 2010. 2. The complexity of gluten proteins and analytical challenges for the accurate quantitation of gluten-free products. 3. The preparation of incurred food samples in a test kitchen facility and the brewing of barley-beer in a pilot-scale plant. 4. A comparison and examination of the efficacy and efficiency of the R5 enzyme-linked immunosorbent assay (ELISA) kits, the RIDASCREEN® Gliadin sandwich and RIDASCREEN® Gliadin competitive assays for the quantification of gluten from wheat, rye, and barley in food samples. 5. An examination into the fitness of the label-free targeted liquid chromatography- tandem mass spectrometry (LC-MS/MS) method for the quantification of gluten from wheat, rye, and barley in food samples.