Browsing by Author "Du Toit, Elbe"

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    The role of the biological soil-root interface in seasonal variation of N and P recycling for Aspalathus linearis
    (Stellenbosch : Stellenbosch University, 2023-03) Du Toit, Elbe; Valentine, A. J. ; Kleinert, Aleysia ; Lotter, Daleen; Stellenbosch University. Faculty of Science. Dept. of Botany and Zoology.
    ENGLISH ABSTRACT: Aspalathus linearis is found in the diverse Cape Floristic Region, known for its nutrient poor soil with a low pH and weather conditions that consists of dry, hot summers and cold, wet winters. Aspalathus linearis is well adapted to the soil in this region by producing cluster roots, forming symbiotic relationships with nitrogen fixing bacteria and by increasing the soil pH through OH- secretion in the roots. Few studies have investigated the seasonal variation in A. linearis physiology with regards to N and P acquisition. This study aims to determine the seasonal variation in N and P acquisition for A. linearis grown in two different soils and determine the biological activities in the soil associated with A. linearis. It is expected that an increase in N and P enzyme activity will be seen for soil with higher nutrient levels. Two soils were identified, one with higher P and C concentrations and a higher clay percentage. Nitrogen and P acquisition was determining using enzyme assays, including GS, NR, GDH-D, GDH- A, Phytase, APase, RNAse and Pi. Soil chemical and physical properties were investigated to determine the differences in the soil and microbial community diversity were investigated in the study. The differences in soil showed a great impact on soil enzyme activity on enzymes such as NR and APase, while having little effect on other soil enzymes. Seasonal growth caused a significant increase in bacterial activity and diversity for both soils identified and an increase in wetter season was found in microbial diversity. Soil conditions such as higher C and P did not show a consistent impact on plant enzyme activity. Enzymes such as GS, GDH-A, GDH-D and Pi were affected by soil conditions, however, seasons affected activity more than soil. Amino acid concentrations increased in winter, with soil in higher C and P having significantly lower concentrations than amino acid concentrations from all winter material measured. The study found that an increase in nutrients including C and P does not lead to an overall increase in N and P acquisition and that enzyme activity varied in dependency on soil conditions. Further studies should investigate the effect of different soil conditions on drought for A. linearis.